Basic Study
Copyright ©The Author(s) 2020.
World J Gastroenterol. Jun 14, 2020; 26(22): 3034-3055
Published online Jun 14, 2020. doi: 10.3748/wjg.v26.i22.3034
Table 1 Primers for reverse transcription quantitative polymerase chain reaction
PrimerForward (5’-3’)Reverse (5’-3’)
miR130a-3pGATGCTCTCAGTGCAATGTTATATGGTTGTTCTGCTCTCTGTCTC
U6ATTGGAACGATACAGAGAAGATTGGAACGCTTCACGAATTTG
hsa_circRNA_102610ACAGTGTGCCAAGTTACTCGAGTGCAAGATAAAGGCCCAA
β-actinGTGGCCGAGGACTTTGATTGCCTGTAACAACGCATCTCATATT
Table 2 Sequences of RNA oligo and probes
RNASequence (5'-3')
hsa_circRNA_102610 si-1GCUGAUUUUUCAGGGUCAATTUUGACCCUGAAAAAUCAGCTT
hsa_circRNA_102610 si-2UUCAGGGUCAACUUUGUAGTTCUACAAAGUUGACCCUGAATT
hsa-miR-130a-3p mimicsCAGUGCAAUGUUAAAAGGGCAUGCCCUUUUAACAUUGCACUGUU
hsa-miR-130a-3p inhibitorAUGCCCUUUUAACAUUGCACUG
hsa_circRNA_102610 probeFAM-GCACACTACAAAGTTGTTGACCCTGAAAAATCAGCAGAA
hsa-miR-130a-3p probeCY3-ATGCCCTTTTAACATTGCACTG