Dynamic changes of key metabolites during liver fibrosis in rats

Table 1 The most prominent metabolites

No.	Ret. time	m/z	Adduct	Compound	Independent t-test
					Con vs Week 1	Con vs Week 4	Con vs Week 8	Con vs Week 12
1	18.6	786.6003	[M+H]+	PC(18:0/18:2)	0.045	0.005	0.001	0.338
2	18.47	810.5999	[M+H]+	PC(18:0/20:4)	0.000	0.000	0.000	0.000
3	18.34	760.5851	[M+H]+	PC(16:0/18:1)	0.130	0.206	0.000	0.363
4	18.29	834.6003	[M+H]+	PC(18:0/22:6)	0.000	0.002	0.071	0.000
5	17.73	758.5689	[M+H]+	PC(16:0/18:2)	0.499	0.526	0.001	0.000
6	17.61	782.569	[M+H]+	PC(18:2/18:2)	0.000	0.000	0.001	0.000
7	17.49	806.5689	[M+H]+	PC(22:6/16:0)	0.000	0.037	0.808	0.439
8	17.31	782.5693	[M+H]+	PC(16:0/20:4)	0.021	0.000	0.000	0.000
9	17.2	806.5676	[M+H]+	PC(18:2/20:4)	0.000	0.041	0.000	0.000
10	17.13	756.5521	[M+H]+	PC(16:1/18:2)	0.477	0.033	0.003	0.000
11	12.94	524.3707	[M+H]+	LysoPC(18:0)	0.000	0.000	0.000	0.001
12	12.62	524.3714	[M+H]+	LysoPC(18:0)	0.000	0.001	0.003	0.335
13	11.62	496.3383	[M+H]+	LysoPC(16:0)	0.170	0.000	0.000	0.000
14	11.6	991.6714	[2M+H]+	LysoPC(16:0)	0.003	0.639	0.789	0.000
15	11.03	544.3396	[M+H]+	LysoPC(20:4)	0.000	0.000	0.000	0.003
16	10.75	544.3382	[M+H]+	LysoPC(20:4)	0.000	0.000	0.000	0.000
17	9.12	373.2716	M+H	Cervonoyl ethanolamide	0.035	0.009	0.000	0.000
18	9.11	817.5807	2M+H	β-muricholic acid	0.030	0.041	0.001	0.004
19	3.34	188.0702	M+H	Indoleacrylic acid	0.253	0.009	0.000	0.000
20	2.71	120.0802	M+H	L-threonine	0.026	0.000	0.000	0.000
21	0.97	120.0799	M+H	Hydroxyethyl glycine	0.008	0.007	0.000	0.000

Table 2 Summary of pathway analysis results

Pathway name	Total	Expected	Hits	Raw P	-LOG(p)	Holm adjust	FDR	Impact
Glycerophospholipid metabolism	30	0.10699	2	0.004255	5.4597	0.34463	0.34463	0.18333
Linoleic acid metabolism	5	0.017832	1	0.01773	4.0325	1	0.71807	0
Alpha-linolenic acid metabolism	9	0.032097	1	0.031732	3.4504	1	0.85677	0
Glycine, serine, and threonine metabolism	32	0.11412	1	0.10918	2.2148	1	1	0
Arachidonic acid metabolism	36	0.12839	1	0.12213	2.1027	1	1	0
Tryptophan metabolism	41	0.14622	1	0.1381	1.9798	1	1	0
Aminoacyl-tRNA biosynthesis	67	0.23894	1	0.21745	1.5258	1	1	0

FDR: False discovery rate.