Long noncoding RNA HOXA11-AS promotes gastric cancer cell proliferation and invasion via SRSF1 and functions as a biomarker in gastric cancer

Table 1 Clinicopathological characteristics of the study population

	Tissue samples	Serum samples	Healthy controls
	(n = 25)
Gender
Male	15	57	26
Female	10	37	14
Age
Mean ± SD	60 ± 7	59 ± 7	59 ± 8
Median (range)	61 (45-72)	60 (40-74)	60 (41-74)
Tumor size (cm)
<5	19	59
≥5	6	35
TNM stage
I	13	18
II	9	21
III	3	42
IV	0	13
Differentiation
Well and moderate	13	50
Poor	12	44
Invasion depth
T1	3	9
T2	7	32
T3	12	35
T4	3	18
Lymph node metastasis
Positive	18	50
Negative	7	44

Table 2 Association of serum HOXA11-AS with clinicopathological characteristics in patients with gastric cancer

Clinical feature	Cases	Serum HOXA11-AS	P-value
Gender			0.824
Male	57	0.345 ± 0.104
Female	37	0.349 ± 0.091
Age (yr)			0.304
<60	38	0.337 ± 0.111
≥60	56	0.355 ± 0.089
Tumor size (cm)			0.001
<5	59	0.320 ± 0.094
≥5	35	0.391 ± 0.092
TNM stage			0.001
I and II	39	0.250 ± 0.073
III and IV	55	0.415 ± 0.040
Differentiation			0.934
Well and moderate	50	0.346 ± 0.112
Poor	44	0.347 ± 0.084
Invasion depth			0.867
T1 and T2	41	0.348 ± 0.100
T3 and T4	53	0.345 ± 0.099
Lymph node metastasis			0.011
Positive	50	0.377 ± 0.088
Negative	44	0.312 ± 0.100

Table 3 Univariate and multivariate survival analyses of 94 gastric cancer patients

Variable	Univariate analysis			Multivariate analysis
	HR	95%CI	P-value	HR	95%CI	P-value
Gender (Male vs Female)	1.310	0.749-2.291	0.344	- - -	- - -	- - -
Age (yr) (<60 vs ≥60)	0.987	0.560-1.739	0.963	- - -	- - -	- - -
Tumor size (cm) (<5 vs ≥5)	0.527	0.301-0.924	0.025	0.816	0.442-1.508	0.516
TNM stage (I + II vs III + IV)	0.195	0.092-0.417	0.001	0.437	0.125-1.522	0.193
Differentiation (Well and moderate vs Poor)	0.923	0.529-1.61	0.779	- - -	- - -	- - -
Invasion depth (T1 + T2 vs T3 vs T4)	1.050	0.601-1.836	0.863	- - -	- - -	- - -
Lymph node metastasis (Positive vs Negative)	0.667	0.379-1.176	0.162	- - -	- - -	- - -
Serum HOXA11-AS (High vs Low)	0.201	0.104-0.385	0.001	0.338	0.115-0.989	0.048