Relaxin influences ileal muscular activity through a dual signaling pathway in mice

Table 1 Boltzmann parameters of I_Ca,L activation and inactivation in a smooth muscle cell in control (Ctr), in the presence of relaxin (RLX) alone or RLX with ODQ (ODQ + RLX), 9CPA (9CPA + RLX) and ODQ + 9CPA (ODQ + 9CPA + RLX)

Parameters	Ctr	RLX	ODQ + RLX	9CPA + RLX	ODQ + 9CPA + RLX
ICa,L/Cm (pA/pF)	8.8 ± 0.5	7.2 ± 0.7a	7.9 ± 0.6	8.2 ± 0.6	8.7 ± 0.6c
Vp (mV)	17.5 ± 2	20.1 ± 2	17.9 ± 2	18.1 ± 1	17.5 ± 1.9
tp (ms)	270.8 ± 22	194.1 ± 19b	230.8 ± 15ac	244.8 ± 22c	259.8 ± 24c
Va (mV)	4.0 ± 0.3	8.9 ± 0.6e	6.0 ± 0.6bc	4.9 ± 0.5ad	4.3 ± 0.4d
ka (mV)	6.5 ± 0.4	6.0 ± 0.5	6.2 ± 0.4	6.4 ± 0.4	6.4 ± 0.4
Vrev (mV)	67.2 ± 4	68.8 ± 4	68.1 ± 5	67.5 ± 4	67.8 ± 4
Vh (mV)	-14.4 ± 2	-10.9 ± 1a	-12.4 ± 1	-12.6 ± 2	-14.0 ± 1c
kh (mV)	7.6 ± 0.5	7.5 ± 0.5	7.6 ± 0.6	7.6 ± 0.7	7.6 ± 0.6
I2h (Norm)	0.09 ± 0.02	0.23 ± 0.02b	0.14 ± 0.01ad	0.13 ± 0.03d	0.09 ± 0.03f
V2h (mV)	22.0 ± 2	22.3 ± 1.9	21.8 ± 2.1	21.5 ± 2	21.9 ± 1.8
k2h (mV)	8.0 ± 0.7	6.3 ± 0.6a	7.1 ± 0.7	7.5 ± 0.3c	7.9 ± 0.2c

In the U shaped inactivation curves (h) the best fit to the data is obtained by the sum of two Boltzmann terms whose parameters are indicated with the subscript h for the term at negative potential, and 2h for the term at positive potential; the amplitude of the normalized second term, I_2h (Norm), was evaluated at 50 mV.

^aP < 0.05,

^bP < 0.01 and

^eP < 0.001 RLX compared to the related control;

^cP < 0.05,

^dP < 0.01 and

^fP < 0.001 compared to RLX. Data for Ctr ( = 67 cells, 25 strips from 12 mice), for RLX (n = 20 cells, 10 strips from 5 mice), for ODQ + RLX (n = 18 cells, 9 strips from 5 mice), for 9CPA + RLX (n = 15 cells, 8 strips from 5 mice) and for ODQ + 9CPA + RLX (n = 16 cells, 9 strips from 5 mice), RLX: Relaxin; ODQ: Oxadiazolo[4,3-a]-quinoxalin-1-one; 9CPA: 9-cyclopentyladenine.