Potential involvement of heat shock proteins in pancreatic-duodenal homeobox-1-mediated effects on the genesis of gastric cancer: A 2D gel-based proteomic study

Table 1 Primer sequences of pancreatic-duodenal homeobox-1 (PDX1) and heat shock protein (HSP) genes used in the RT-PCR analysis

Gene name	Forward	Reverse	Product size (bp)
PDX1	5’-ATCTCCCCATACGAAGTGCC-3’	5’-CGTGAGCTTTGGTGGATTTCAT-3’	92
GAPDH	5’-ATGGGGAAGGTGAAGGTCG-3’	5’-GGGGTCATTGATGGCAACAATA-3	108
HSPA2	5’-CACCACCTATTCGTGCGTC-3’	5’-TTTCCGTCCAATCAGCCTCTT-3’	196
HSPA6	5’-CAAGGTGCGCGTATGCTAC-3’	5’-GCTCATTGATGATCCGCAACAC-3’	224
HSPA8	5’-GGAGGTGGCACTTTTGATGTG-3’	5’-CAAGCAGTACGGAGGCGTCT-3’	200
HSPA1B	5’-TTTGAGGGCATCGACTTCTACA-3	5’-CCAGGACCAGGTCGTGAATC-3’	148
HSPA1L	5’-CTACTGCCAAGGGAATCGCC-3’	5’GCCGATCAGACGTTTAGCATC-3’	227
HSP27	5’-GGACGAGCATGGCTACATCT-3’	5’-CTTTACTTGGCGGCAGTCTC-3’	237
HSP60	5’-CACCGTAAGCCTTTGGTCAT-3’	5’-CCCTCTTCTCCAAACACTGC-3’	188

Table 2 Differential protein spots between SGC7901 with pcDNA3.1(+)-pancreatic-duodenal homeobox-1 (PDX1) vector and SGC7901-pcDNA cells analyzed by matrix-assisted laser desorption ionization-time of flight-mass spectrometry

Spot No.	Rank protein name	Accession No.	Protein score	Protein score CI%	Protein MW
S1	Heat shock 70 kDa protein 8 isoform 2 (homo sapiens)	gi 24234686	595	100	53598.4
	HSPA8 protein (homo sapiens)	gi 48257068	546	100	54804.2
	Heat shock 70 kDa protein 2 (homo sapiens)	gi 3287 9973	290	100	70263.0
	Heat shock 70 kDa protein 6 (HSP70B’) (homo sapiens)	gi 55960611	81	100	71440.4
S2	DNAK-type molecular chaperone HSPA1L-human	gi 2119712	559	100	70110.0
	HSPA1A protein (homo sapiens)	gi 14414588	558	100	70294.1
	Heat shock 10 kDa protein 1-like (homo sapiens)	gi 55961919	372	100	70730.5
	Heat shock 70 kDa protein 1-like (homo sapiens)	gi 21759781	361	100	70748.4
	Heat shock 70 kDa protein 1B (homo sapiens)	gi 55962554	296	100	52199.8
S3	Chaperonin 60 (Hsp60) (homo sapiens)	gi 6996447	375	100	61187.4
S4	ER-60 protein (homo sapiens)	gi 2245365	223	100	57146.9
	Glucose regulated protein 58 kDa (Bos taurus)	gi 27805905	113	100	57293.0
S5	Ribosomal protein SA (laminin receptor 1) (homo sapiens)	gi 47125390	204	100	32933.5
S6	PREDICTED: Similar to epsilon isoform of 14-3-3 protein	gi 57091321	53	37.851	29326.5
S7	Heat shock 70 kDa protein 1B (homo sapiens)	gi 55962554	67	97.468	52199.8