Mechanism of combined use of vitamin D and puerarin in anti-hepatic fibrosis by regulating the Wnt/β-catenin signalling pathway

Table 1 Designations and sequences of primers

Designation (mice)	Sequence (5’-3’)
GAPDH	F: GGCATCCTGACCCTCAAGTA
	R: GGGGTGTGAACCTCTCAAA
Collagen I	F: GGACACTACTGGATCGACCTAAC
	R: CTCACCTGTCTCCATGTTGCA
Collagen III	F: CTACCTTGCTCAGTCCTATGAGTCTAGA
	R: TCCCGAGTCGCAGACACATAT
Wnt1	F: GAAACCGCCGCTGGAACT
	R: CCCTGCCTCGTTATTGTGAAG
β-catenin	F: ACC TCC CAAGTC CTG TAT
	R: CCT GGT CCT CGT CAT TTA

Table 2 Biochemical indices of blood and liver

Groups	HA (μg/L)	Hyp (μg/g)
C	61 ± 20.6bcde	195.6 ± 10.5bcde
CCl4	157.3 ± 44.3acde	503 ± 31.7acde
V	70.2 ± 12.9b	375.2 ± 26.9ab
P	79.5 ± 11.6b	361.3 ± 24.1ab
V + P	65 ± 12.1b	353.7 ± 21.6ab

In the comparison of the mean ± standard deviation (SD) of various groups, differences are statistically significant when P < 0.05, with n = 10. In addition, a, b, c, d, and e represent statistical differences vs C, CCl₄, V, P, and V + P groups, respectively. C: Control; V: Vitamin; P: Puerarin.

Table 3 The mRNA levels of collagen I, collagen III, Wnt1, and β-catenin of liver

Groups	Collagen I	Collagen III	Wnt1	β-catenin
C	1.17 ± 0.16bcde	0.62 ± 0.15bcde	0.56 ± 0.13bcde	0.48 ± 0.21bcde
CCl4	4.73 ± 0.76acde	2.47 ± 1.12acde	1.77 ± 0.32acde	2.16 ± 0.42acde
V	1.97 ± 0.31b	1.29 ± 0.19ab	1.06 ± 0.35ab	1.25 ± 0.33ab
P	1.75 ± 0.46b	1.35 ± 0.17ab	0.83 ± 0.19ab	0.71 ± 0.46ab
V + P	1.53 ± 0.12abcd	1.03 ± 0.08abcd	0.61 ± 0.24abcd	0.59 ± 0.14abcd

Differences are considered to exhibit statistical significance when P < 0.05 (n = 10) in the comparison of the mean ± standard deviation (SD) between various groups. a, b, c, d, and e indicate that there are statistical differences vs C, CCl₄, V, P, and V + P groups, respectively. C: Control; V: Vitamin; P: Puerarin.