Reabsorption of iron into acutely damaged rat liver: A role for ferritins

Table 1 Primers used for polymerase chain reaction

Primer	5’ → 3’ Forward	5’ → 3’ Reverse
UBC	CAC CAA GAA GGT CAA ACA GGA A	AAG ACA CCT CCC CAT CAA ACC
Ferritin H	GCCCTGAAGAACTTTGCCAAAT	TGCAGGAAGATTCGTCCACCT
Ferritin L	AACCACCTGACCAACCTCCGTA	TCAGAGTGAGGCGCTCAAAGAG
IL-6	GTCAACTCCATCTGCCCTTCAG	GGCAGTGGCTGTCAACAACAT
IL-1β	TACCTATGTCTTGCCCGTGGAG	ATCATCCCACGAGTCACAGAGG
TNF-α	ACAAGGCTGCCCCGACTAT	CTCCTGGTATGAAGTGGCAAATC
IFN-γ	GAACTGGCAAAAGGACGGTA	CTGATGGCCTGGTTGTCTTT

Table 2 Antibodies used in this study

Antibodies	Company	Reference	Dilution
FTH	Santa Cruz	SC-25617	1:100
FTH	LS Bio	LS-C23537	1:250
FTL	Abcam	AB-69090	1:1000
FTL	Santa Cruz	SC-14422	1:100
FTL peptide	Santa Cruz	SC-14422	1:50
β-actin	Sigma	A-2228	1:4000