Yangzheng Sanjie decoction regulates proliferation and apoptosis of gastric cancer cells by enhancing let-7a expression

Table 1 Clinical and pathological characteristics of gastric cancer patients

Characteristic	n (%)
Age (yr)
< 60	6 (54.5)
≥ 60	5 (45.5)
Sex
Male	8 (72.7)
Female	3 (27.3)
Lymph node metastasis
Negative	6 (54.5)
Positive	5 (45.5)
Histopathology
Adenocarcinoma	11 (100)
Others	0 (0)
Grade
Well/moderately differentiated	5 (45.5)
Poorly differentiated	6 (54.5)
Stage
Early	2 (18.2)
Advanced	9 (81.8)
Surgical therapy
Negative	0 (0)
Positive	11 (100)

Table 2 Differentially expressed microRNAs in gastric cancer cells treated with Yangzheng Sanjie decoction

miRNA ID	Fold change (YZSJD/control)
	AGS	HS-746T
hsa-let-7a-5p	1.54	1.80
hsa-let-7d-3p	1.66	1.53
hsa-let-7f-5p	1.68	2.70
hsa-miR-7-5p	1.56	2.03
hsa-miR-15a-5p	1.66	2.04
hsa-miR-15b-5p	1.62	3.26
hsa-miR-21-3p	1.86	1.75
hsa-miR-22-3p	1.58	1.78
hsa-miR-22-5p	1.74	1.60
hsa-miR-25-3p	1.74	1.76
hsa-miR-26b-5p	1.64	1.80
hsa-miR-27a-3p	2.07	1.59
hsa-miR-27b-3p	1.55	1.66
hsa-miR-30a-5p	1.71	4.65
hsa-miR-30b-5p	1.55	1.73
hsa-miR-30d-5p	1.67	1.98
hsa-miR-30e-3p	1.52	1.77
hsa-miR-32-5p	1.60	1.83
hsa-miR-92b-3p	1.58	1.95
hsa-miR-98-5p	1.51	1.55
hsa-miR-99a-5p	1.73	1.56
hsa-miR-105-5p	1.57	2.59
hsa-miR-125a-5p	1.67	1.72
hsa-miR-139-5p	2.35	1.91
hsa-miR-181b-5p	1.78	2.68
hsa-miR-186-5p	1.65	1.71
hsa-miR-192-5p	-2.27	-4.17
hsa-miR-193b-3p	3.33	1.53
hsa-miR-212-3p	1.81	1.67
hsa-miR-215-5p	-2.94	-4.76
hsa-miR-361-5p	1.83	1.84
hsa-miR-374a-5p	1.83	2.06
hsa-miR-450a-5p	1.59	1.65
hsa-miR-452-5p	1.61	2.58
hsa-miR-454-3p	1.59	2.44
hsa-miR-484	1.65	2.18
hsa-miR-514a-3p	1.60	3.34

miRNAs with a fold change ≥ 1.5 in both cell lines are listed. YZSJD: Yangzheng Sanjie decoction; miRNAs: MicroRNAs.

Table 3 Let-7a and c-Myc expression in gastric cancer tissue samples

Tissue	Let-7a					c-Myc
	(-)	(+)	(++)	(+++)	P value	(-)	(+)	(++)	(+++)	P value
Ca	3	7	1	0	< 0.05	0	2	4	5	< 0.05
Pc	0	8	3	0		1	6	3	1
N	0	1	6	4		4	6	1	0

Staining intensity was described as negative (-), weak (+), moderate (++) and strong (+++) staining. Ca: Gastric cancer tissues; Pc: Matched para-carcinoma tissues; N: Matched distal normal gastric tissues.