Dietary advanced glycation end-products aggravate non-alcoholic fatty liver disease

Table 1 Primer and probe sequences used for real time qPCR

Gene name	Probe/primer	Sequence
IL-6	Probe	5-FAM ATTGCCATTGCACAACT-3
	Forward	5-GGGAAATCGTGGAAATGAGAAA-3
	Reverse	5-AAGTGCATCATCGTTGTTCATACA-3
MCP1	Probe	5-FAM-AATGGGTCCAGACATAC-3
	Forward	5-GTCTGTGCTGACCCCAAGAAG-3
	Reverse	5-TGGTTCCGATCCAGGTTTTTA-3
TNFα	Probe	5-FAM-TCACCCACACCGTCAG-3
	Forward	5-GGCTGCCCCGACTACGT-3
	Reverse	5-TTTCTCCTGGTATGAGATAGCAAATC-3
RAGE	Probe	5-FAM-CACAGCCCGGATTG-3
	Forward	5-GCTGTAGCTGGTGGTCAGAACA-3
	Reverse	5-CCCCTTACAGCTTAGCACAAGTG-3
alphaSMA	Probe	5-FAM-TGCCAGATCTTTTCC-3
	Forward	5-GACGCTGAAGTATCCGATAGAACA-3
	Reverse	5-GGCCACACGAAGCTCGTTAT-3
COL1A1	Probe	5-FAM-ATCGACCCTAACCAAG-3
	Forward	5-GACTGGAAGAGCGGAGAGTACTG-3
	Reverse	5-CCTTGATGGCGTCCAGGTT-3
CTGF	Probe	5-FAM-C ACTGCCTGGTCCAGAC-3
	Forward	5-GCTGCCTACCGACTGGAAGA-3
	Reverse	5-CTTAGAACAGGCGCTCCACTCT-3

Table 2 Animal characteristics and metabolic findings

	Body weight (g)	Epididymal fat (g)	Liver weight (g)	Blood glucose (mmol/L)	OGTT (AUC)
Normal chow	35.4 ± 1.5a	1.4 ± 0.2a	1.3 ± 0.1a	8.0 ± 0.7	654.1 ± 94.4
HFHC	43.4 ± 1.7	2.7 ± 0.2	3.7 ± 0.4	8.2 ± 0.6	528.7 ± 65.2
HFHC baked	45.5 ± 1.2	2.8 ± 0.2	3.4 ± 0.5	7.8 ± 0.3	506.2 ± 70.2
HFHC baked and acetic acid	44.6 ± 0.9	2.6 ± 0.1	3.6 ± 0.2

Data are mean ± SEM at 33 wk of normal chow or high fat high cholesterol feeding.

^aP < 0.01 vs HFHC groups. AUC: Area under the curve; OGTT: Oral glucose tolerance test.