Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. Sep 21, 2016; 22(35): 8017-8025
Published online Sep 21, 2016. doi: 10.3748/wjg.v22.i35.8017
Table 1 Primers and probes
Tissue transglutaminase 2
Primer Forward:ATAAGTTAGCGCCGCTCTCC
Primer Reverse: CGGTGGCTCCTTCCACTG
Probe: GCCAGCCGCCAGTG
Interferon gamma
Primer Forward: CGCTTTACTTTATAGAAAACCTGGA
Primer Reverse: TCAATGAAGAGAACTTGGTCATTC
Probe: GCTTGAATCTAAA
Toll-like receptor 2
Primer Forward: CAAGATTCAAAGTATTTA
Primer Reverse: CCAGGTG CATTTAAAGA
Probe: TGCCCCTACTCAATCT
MyD88
Primer Forward: CAAGGCCTTGTCCCTGC
Primer Reverse: TCTGCCCTGCCTCCT
Probe: AGGCCCTGGGTGTGTGT
Table 2 Main demographic, clinical and pathologic features of enrolled patients
Celiac disease(n = 31)Gluten sensitivity(n = 17)Irritable bowel syndrome(n = 29)Other ME(n = 12)Negative controls(n = 15)P value
Age34 ± 1234.3 ± 6.136.2 ± 13.434.7 ± 14.232.6 ± 9.50.891
Sex (F/M)19/1215/222/711/19/60.062
IELs count51.6 ± 10.618.6 ± 4.915.5 ± 5.119.0 ± 7.65.3 ± 1.5< 0.0011
Weight loss19 (61.3)7 (41.2)4 (13.8)6 (50)00.0022
Abdominal pain25 (80.6)16 (94.1)28 (96.5)8 (66.6)00.042
Diarrhea21 (67.7)6 (35.3)29 (100)6 (50)0< 0.0012
Weakness14 (45.1)8 (47.0)8 (27.6)3 (25)00.092
Headache2 (6.5)7 (41.2)4 (13.8)0 (0)00.732
DQ 2-829 (93.5)10 (58.8)11 (37.9)0 (0)NA< 0.0012
Iron deficiency anemia8 (25.8)2 (11.8)2 (6.9)8 (66.6)00.632