Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia

doi:10.3748/wjg.v22.i24.5512

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 28, 2016; 22(24): 5512-5519
Published online Jun 28, 2016. doi: 10.3748/wjg.v22.i24.5512

Table 1 Primer sequences for RT-PCR

Primer name	Sequence (5'→3')
mTRPV4-F	ACAACACCCGAGAGAACACC
mTRPV4-R	CCCAAACTTACGCCACTTGT
mGAPDH-F	TGAAGGGTGGAGCCAAAAGG
mGAPDH-R	GGAAGAGTGGGAGTTGCTGTTG
rTRPV4-F	CCTGGCAGGGATCGAGGCCT
rTRPV4-R	GGATGGTGGTGGCCCACTGC
rGAPDH-F	GCCAAGGCTGTGGGCAAGGT
rGAPDH-R	GAGCAATGCCAGCCCCAGCA

Table 2 Primary and secondary antisera for immunochemistry

Tissue antigen/host	Dilution	Source
TRPV4/rabbit	1:500	B. Nilius, or Abcam
Goat anti-rabbit IgG-Alexa488	1:1500	Invitrogen, Inc.

Citation: Mihara H, Suzuki N, Boudaka AA, Muhammad JS, Tominaga M, Tabuchi Y, Sugiyama T. Transient receptor potential vanilloid 4-dependent calcium influx and ATP release in mouse and rat gastric epithelia. World J Gastroenterol 2016; 22(24): 5512-5519
URL: https://www.wjgnet.com/1007-9327/full/v22/i24/5512.htm
DOI: https://dx.doi.org/10.3748/wjg.v22.i24.5512