Chitooligosaccharides promote radiosensitivity in colon cancer line SW480

Table 1 Inhibition of chitooligosaccharides for the proliferation of SW480 cells

COS concentration (mg/mL)	OD (mean ± SD)	Inhibition rate
0	1.019 ± 0.007	-
0.5	0.969 ± 0.0051	4.91%
1.0	0.908 ± 0.0082	10.89%
2.0	0.804 ± 0.0063	21.10%
3.0	0.692 ± 0.0074	32.09%
4.0	0.580 ± 0.0065	43.08%
5.0	0.433 ± 0.0086	57.51%
P	0	-
F	137.6	-

Compared with COS concentrations in the 0 mg/mL Group:

¹P = 0.000, q = 17.437;

²P = 0.000, q = 31.326;

³P = 0.000, q = 80.047;

⁴P = 0.000, q = 99.096;

⁵P = 0.000, q = 142.849;

⁶P = 0.000, q = 165.379. COS: Chitooligosaccharides.

Table 2 Comparison of cells survival rates for different radiation doses between the RAY and RAY + COS group

Irradiation dose (Gy)	Cell survival rate (%)
	RAY	RAY + COS
0	99.22 ± 3.51	99.17 ± 4.061
1	90.67 ± 3.82	85.30 ± 3.382
2	73.69 ± 3.45	56.11 ± 2.953
4	45.95 ± 3.41	28.64 ± 2.764
6	23.84 ± 2.20	12.53 ± 2.035
8	8.68 ± 1.75	3.81 ± 1.166
P	0	0
F	182.7	243.2

Compared with RAY group:

¹P = 0.978, t = 0.028;

²P = 0.006, t = 3.158,

³P = 0.000, t = 11.619;

⁴P = 0.000, t = 11.837;

⁵P = 0.000, t = 11.335;

⁶P = 0.000, t = 6.959. COS: Chitooligosaccharides.

Table 3 Comparison of cell apoptosis rates among the three groups

Groups	Cell apoptosis rate
Non-treatment group	1.79 ± 0.37
RAY group	9.33 ± 1.051
RAY + COS group	22.64 ± 1.2723

Compared with the control group:

¹P = 0.000, t = -20.318;

²P = 0.000, t = -47.286; compared with the RAY Group:

³P = 0.000, t = -24.232. COS: Chitooligosaccharides.

Table 4 Comparison of cell cycle distribution among the three groups

Groups	S (%)	G0/G1 (%)	G2/M (%)
Non-treatment group	30.15 ± 0.82	39.41 ± 1.05	30.44 ± 0.68
RAY group	22.48 ± 0.741	30.51 ± 0.862	47.01 ± 0.523
RAY + COS group	18.89 ± 0.6547	24.34 ± 0.4658	56.77 ± 0.2869

Compared with the control group:

¹P = 0.000, t = 20.832;

²P = 0.000, t = 19.672;

³P = 0.000, t = -58.070;

⁴P = 0.000, t = 32.283;

⁵P = 0.000, t = 39.438;

⁶P = 0.000, t = -107.412; compared with the RAY group:

⁷P = 0.000, t = 10.935;

⁸P = 0.000, t = 18.979;

⁹P = 0.000, t = -49.577. COS: Chitooligosaccharides.