Basic Study
Copyright ©The Author(s) 2016.
World J Gastroenterol. May 21, 2016; 22(19): 4695-4706
Published online May 21, 2016. doi: 10.3748/wjg.v22.i19.4695
Table 1 Primer sequences
E-cadherinForward 5′-CACACTGATGGTGAGGGTACAAGG-3′
Reverse 5′-GGGCTTCAGGAACACATACATGG-3′
VimentinForward 5′-ACCGCTTCGCCAACTACATC-3′
Reverse 5′-GCAACTCCCTCATCTCCTCCT-3′
FibronectinForward 5′-GACTCGCTTTGACTTCACCAC-3′
Reverse 5′-ATCTCCTTCCTCGCTCAGTTC-3′
TGF-β1Forward 5′-GAGGCGGTGCTCGCTTTGTA-3′
Reverse 5′-GCACTGCTTCCCGAATGTCTG-3′
Smad3Forward 5′-ATACGGATGTTCAAGTGTTCG-3′
Reverse 5′-ACTGGGTCCTCTTTGGTTTT-3′
SnailForward 5′-GTCCTTGCTCCACAAACACCA-3′
Reverse 5′-CTGCCTTCCATCAGCCATCT-3′
OccludinForward 5′-AGATGCTGGTTGCTGGAGAAGT-3′
Reverse 5′-TGGAGACAGGAAACGGATGGT-3′
ZO-1Forward 5′-CGGAGCAGAGAGGAAGAGC-3′
Reverse 5′-GGCAGAACACCATCAGAAGG-3′
Claudin-1Forward 5′-AGGTCTGGCGACATTAGTGG-3′
Reverse 5′-TGGTGTTGGGTAAGAGGTTG-3′
GAPDHForward 5′-TGAGGACCAGGTTGTCTC-3′
Reverse 5′-TCCACCACCCTGTTGCTGTA-3′