Genetic variation of occult hepatitis B virus infection

Table 1 Summary of hepatitis B virus surface antigen variants involved in functional studies

Variants	Source	Model	Antigenicity	Immuno-genicity	HBsAg Intra-	HBsAgExtra-	Viral replication/secretion	References
W741	CHB	Huh7	L		L	L		[65]
M75T		Huh7			↑	↓	↓2	[75]
Y100C	OBI	Huh7	↑					[76]
Y100S	OBI	Huh7	L					[77]
I110M		Huh7					L3	[78]
T114R		Huh7					↓3	[79]
T115A		Huh7					↓3	[79]
T116N	OBI	Huh7	↓					[77]
G119E		Huh7				↓	↓3	[78]
G119R	OBI	Huh7/mice	↓		↑	↓	↓3	[66]
P120T	OBI	Huh7/mice	↓					[66]
P120T		Huh7				↓		[79]
C121S		HepG2/Hela	↓		↓	↓		[69]
C1211	OBI	Pichia	↓ or L					[80]
C121A		Huh7				↓	↓ or L3	[79]
K122I		HepG2/Hela	↓		↓	↓		[69]
K122I		Huh7	↓	↓	↓	↓		[81]
K122I		Huh7/Hela	↓ or L	↓	↓	↓		[82]
R122P	OBI	Huh7	↓					[77]
T123N		HepG2/Hela	↓		↓	↓		[69]
T123N		Huh7/Hela	↓ or L				↓3	[82]
C1241	OBI	Pichia	↓ or L					[80]
C124A		Huh7				↓	↓ or L3	[79]
C124R	OBI	Huh7/mice	↓				↓3	[66]
C124Y	OBI	Huh7/mice	↓				↓3	[66]
T125A	CHB	Huh7	↓			↓		[65]
I126S	OBI	Huh7/mice			↑	↓	↓3	[66]
P127S		Huh7					↓3	[79]
Q129H		Huh7					↓3	[79]
Q129R	OBI	Huh7/mice			↑	↓	↓3	[66]
G130R		Huh7					L3	[79]
T131I		Yeast	↓					[83]
M133T4		Huh7						[78]
S136P	OBI	Huh7/mice			↑	↓	↓3	[66]
C1371	OBI	Pichia	↓ or L					[80]
C138Y		Huh7				↓	L3	[79]
C139R	CHB	HepG2	↓					[84]
C139R	OBI	Huh7/mice	↓				↓3	[66]
T140I	OBI	Huh7/mice			↑	↓	↓3	[66]
K141E	OBI	Huh7/mice	↓		↑	↓	↓3	[66]
K141E		Huh7				↓	↓3	[79]
K141E		Yeast	↓					[83]
P142S		Yeast	↓					[83]
P142S		Huh7				↓		[79]
P142A	KT	Huh7	↓					[85]
D144V	KT	Huh7	↓					[85]
D144A	OBI	Huh7/mice	↓		↑	↓	↓3	[66]
D144G		Huh7				↓	L3	[79]
D144E	LC	HepG2	↓					[84]
G145R		Yeast	↓					[83]
G145R		HepG2/Hela	↓		↓	↓		[69]
G145R	LT	Huh7					↓3	[67]
G145R		Huh7	↓	↓		↓		[81]
G145R		Huh7				↓		[79]
G145R	OBI	Huh7/mice	↓		↑	↓	↓3	[66]
G145A	OBI	Huh7/mice	↓					[66]
G145A	OBI	Huh7/HepG2	↓		↑	↓		[83]
G145A		Yeast	↓					[81]
G145W		Huh7		↓		↓		[81]
G145I		Huh7	↓	↓				[81]
G145P		Huh7	↓					[81]
G145N		Huh7	↓					[81]
G145D		Huh7	↓					[81]
G145E		Huh7		↓				[79]
N146S		Huh7					L3	[79]
C147A		Huh7					↓ or L3	[79]
C147R		Huh7				↓	↓3	[79]
C149A		Huh7				↓	↓ or L3	[79]
C149R		Huh7				↓	L3	[79]
A159G		Huh7/Hela	↓	↓			↓3	[82]
K160N		Huh7/Hela	↓	↑5			↓3	[82]
R169P/G		Huh7				↓	L3	[78]
Y100S + S143L	OBI	Huh7	L					[77]
M103I + G145A	OBI	Huh7/HepG2	↓		↓	↓		[86]
M103I + R122K + G145A	OBI	Huh7/HepG2	↓		↓	↓		[86]
T115I + T116N	CHB	HepG2	↓ or L					[87]
T116N + S143L	OBI	Huh7	↓					[77]
R122P + Q101R	OBI	Huh7	↓					[77]
R122P + S167L	OBI	Huh7	↓					[77]
R122K + G145A	OBI	Huh7/HepG2	↓		↓	↓		[86]
P142A + D144V	KT	Huh7	↓					[85]
A159G + K160N		Huh7/Hela	↓	↑5			↓3	[82]

¹Stop codon mutation;

²Viral replication;

³Viral secretion;

⁴Correct that I110M, G119E, G145R, N146Q/S, R169H mutations lead to the decrease of viral secretion;

⁵Cell-mediated immunity. ↑: Increase; ↓: Decrease. Intra-HBsAg: Intracellular HBsAg; Extra-HBsAg: Extracellular HBsAg; L: Dramatic decrease; CHB: Chronic hepatitis B; OBI: Occult HBV infection; LC: Liver cirrhosis; KT: Kidney transplant; LT: Liver transplant; HBsAg: Hepatitis B virus surface antigen.