Basic Study
Copyright ©The Author(s) 2015.
World J Gastroenterol. Aug 7, 2015; 21(29): 8858-8867
Published online Aug 7, 2015. doi: 10.3748/wjg.v21.i29.8858
Table 1 Effect of lipopolysaccharide on hepatic stellate cells-T6 proliferation assessed by methyl thiazoyltetrazolium assay
Parameter24 h (OD)48 h (OD)
Control0.5033 ± 0.0230.8853 ± 0.021
LPS (10 ng/mL)0.5998 ± 0.019ab0.9813 ± 0.037ab
LPS (100 ng/mL)0.6002 ± 0.038ab1.1126 ± 0.050ab
LPS (1000 ng/mL)0.7032 ± 0.066b1.2469 ± 0.0525b
LPS (5000 ng/mL)0.6625 ± 0.015b1.2141 ± 0.018b
Table 2 Effect of ablation of pim-3 on HSC-T6 cell proliferation assessed by methyl thiazoyltetrazolium assay
Group24 h (OD)48 h (OD)
Control0.5267 ± 0.0300.8435 ± 0.028
Liposomes0.5749 ± 0.0280.8552 ± 0.014
si-pim30.2987 ± 0.050b0.4634 ± 0.056b
scrambled0.5062 ± 0.0660.8069 ± 0.039
si-pim3 plus LPS0.4063 ± 0.051ab0.5434 ± 0.031b
LPS0.7435 ± 0.028b1.2136 ± 0.048bc
Table 3 Effect of ablation of pim-3 on caspase-3 activity of hepatic stellate cells-T6 cells
GroupsCaspase-3 activity (U)
Control5.12 ± 1.33
Liposomes4.95 ± 1.62
si-pim313.24 ± 2.81bd
scrambled5.28 ± 0.93
si-pim3 plus LPS11.35 ± 0.85bd
LPS3.21 ± 0.55a