Sophocarpine attenuates liver fibrosis by inhibiting the TLR4 signaling pathway in rats

Table 1 Primers used for detection of gene transcription

Gene	Forward	Reverse
α-SMA	5'-ACTGGGACGACATGGAAAAG-3'	5'-CATCTCCAGAGTCCAGCACA-3'
Pro-collagen I	5'-AGGCATAAAGGGTCATCGTG-3'	5'-ACCGTTGAGTCCATCTTTGC-3'
Collagen III	5'-GTCCACGAGGTGACAAAGGT-3'	5'-CATCTTTTCCAGGAGGTCCA-3'
TNFα	5'-AGATGTGGAACTGGCAGAGG-3'	5'-CCCATTTGGGAACTTCTCCT-3'
IL-6	5'-CCGGAGAGGAGACTTCACAG-3'	5'-ACAGTGCATCATCGCTGTTC-3'
TGFβ1	5'-ATACGCCTGAGTGGCTGTCT-3'	5'-TGGGACTGATCCCATTGATT-3'
MCP-1	5'-ATGCAGTTAATGCCCCACTC-3'	5'-TTCCTTATTGGGGTCAGCAC-3'
TLR4	5'-TGCTCAGACATGGCAGTTTC-3'	5'-TCAAGGCTTTTCCATCCAAC-3'
Myd88	5'-GAGATCCGCGAGTTTGAGAC-3'	5'-CTGTTTCTGCTGGTTGCGTA-3'
TRAF6	5'-AGGGTACAATACGCCTCACG-3'	5'-GCGGGTAGAGACTTCACAGC-3'
ERK1	5'-TCCAAGGGCTACACCAAATC-3'	5'-AGGTAGTTTCGGGCCTTCAT-3'
JNK1	5'-GCCACAAAATCCTCTTTCCA-3'	5'-CACATCGGGGAACAGTTTCT-3'
Cyclin D1	5'-GCGTACCCTGACACCAATCT-3'	5'-GGCTCCAGAGACAAGAAACG-3'
β-actin	5'-GCCAACACAGTGCTGTCTGG-3'	5'-TGATCCACATCTGCTGGAAGG-3'

Table 2 Effect of sophocarpine on the improvement in serum alanine aminotransferase, aspartate aminotransferase and total bilirubin levels in both models of liver fibrosis

Group	ALT (U/L)	AST (U/L)	TB (mmol/L)
Normal	29.29 ± 1.76	123.67 ± 29.06	1.29 ± 0.28
DMN model	68.27 ± 3.43	176.9 ± 8.99	8.09 ± 1.35
DMN + sophocarpine	48.58 ± 4.521	108.42 ± 15.461	3.00 ± 0.481
Sham operation	28.83 ± 2.22	121.00 ± 36.72	2.00 ± 0.63
BDL model	150.50 ± 23.6	959.50 ± 255	139.67 ± 16.23
BDL + sophocarpine	91.86 ± 6.711	464.14 ± 182.61	123.86 ± 9.69

¹Compared with the model group, P < 0.05. ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; TB: Total bilirubin; DMN: Dimethylnitrosamine; BDL: Bile duct ligation.