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Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Oct 28, 2014; 20(40): 14615-14625
Published online Oct 28, 2014. doi: 10.3748/wjg.v20.i40.14615
Table 1 Comparison between different nucleic acid amplification technologies
MethodTemplate requirementSensitivity/specificityEnzyme(s) requiredTemperature requirementPrimers/primer designMultiplexpossibilityRapid detection possibilityTime to detect in (min)Sensitive to biological inhibitors
PCRdsDNA1, RNAAbout 1-10 copies/Very highDNA polymerase95 °C →55-60 °C →68-72 °C (Cyclic)≥ 2/simpleYesYes2About 40-120Yes
LCRDNA1About 1-10 copies/Very highDNA ligase and DNA polymerase94 °C →65 °C(Cyclic)4/simpleYesYes2About 100-180Yes
LAMPssDNA1, RNAAbout 5 copies/Very highBst DNA polymerase60-65 °C(Isothermal)4-6/complexN/AYes234About 60-90Less
NASBARNA1, DNAAbout 1 copy/Very highReverse transcriptase, T7 RNA polymerase, RNAse H37-41 °C(Isothermal)2/simpleYesYes24About 60-120Yes
RCACircular ssDNA1About 10 copies/high29 DNA polymerase30-65 °C(Isothermal)1/simpleN/AYes2About 60-90Less
TMARNA1, DNAAbout 1-10 copies/highReverse transcriptase, RNA polymerase50-60 °C(Isothermal)2/simpleYesYes2About 120-140Yes
SDAssDNA1, RNA,About 10 copies/lowBst DNA polymerase or exo-Klenow Fragment95 °C →37 °C(Isothermal)4/complexYesYes24About 90-120Yes
tHDAdsDNA1About 1-10 copies/Very highHelicase, DNA polymerase60-65 °C(Isothermal)2/simpleYesYes24About 75-90Less