Original Article
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Jul 21, 2014; 20(27): 9106-9115
Published online Jul 21, 2014. doi: 10.3748/wjg.v20.i27.9106
Table 1 Primer sets used for quantitative reverse transcriptase-polymerase chain reaction analysis of mouse colonic tissue
Gene nameAccession No.Product sizeDirectionSequence (5’ to 3’)Ref.
Claudin2NM_016675.4120 bpForwardGGCGTCCAACTGGTGGGCTAC[42]
ReverseAACCGCCGTCACAATGCTGGC
Claudin3NM_009902.4132 bpForwardCAGGGGCAGTCTCTGTGCGAG[42]
ReverseGCCGCTGGACCTGGGAATCAAC
GAPDHNM_008084.2132 bpForwardAACTTTGGCATTGTGGAAGG[42]
ReverseGGATGCAGGGATGATGTTCT
IL-1βNM_00836173 bpForwardTCGCTCAGGGTCACAAGAAA[43]
ReverseCATCAGAGGCAAGGAGGAAAAC
INFγNM_008337.393 bpForwardAGGTCCAGCGCCAAGCATTCAA[42]
ReverseAGCAGCGACTCCTTTTCCGCTT
iNOSU43428.176 bpForwardCAAAGTCTCAGACATGGCTTGCThis study
ReverseTTCCTCTGTCAGGTCACTTTGG
NOX1NM_172203.1113 bpForwardCAGGCATCCTCATTTTGCGGThis study
ReverseCCTTCTGCTGGGAGCGATAA
T-betNM_019507.2138 bpForwardCCACTGGATGCGCCAGGAAGTT[42]
ReverseTTCACCTCCACGATGTGCAGCC
TNF-αNM_013693.267 bpForwardTGGGACAGTGACCTGGACTGT[43]
ReverseTTCGGAAAGCCCATTTGAGT
Table 2 Primers for quantitative polymerase chain reaction analysis of selected fecal microbiota
Target organismPrimer setSequence (5’ to 3’)Product sizeRef.
BacteroidesBactF285GGTTCTGAGAGGAGGTCCC53[44]
UniR338GCTGCCTCCCGTAGGAGT
Ec-ssulEc-ssu1FGGATAACACTTGGAAACAGG115[45]
Ec-ssu1RTCCTTGTTCTTCTCTAACAA
EubacteriaUniF340ACTCCTACGGGAGGCAGCAGT210[46]
UniR514ATTACCGCGGCTGCTGGC
LactobacillusLabF362AGCAGTAGGGAATCTTCCA315[44]
LabR677CACCGCTACACATGGAG
Ruminococcus albus (Ralb)Ralb561FCAGGTGTGAAATTTAGGGGC246[47]
Ralb807RGTCAGTCCCCCCACACCTAG
Table 3 Organ weights of 10-week-old interleukin-10-deficient and double knockout mice at necropsy
Organ weight (g)IL-10-deficientDKOP value
Liver1.12 ± 0.0411.26 ± 0.030.0098
Heart0.13 ± 0.010.13 ± 0.01NS
Spleen0.09 ± 0.010.16 ± 0.020.0006
Vastus muscle0.24 ± 0.010.23 ± 0.02NS
Gastrocnemius muscle0.27 ± 0.010.25 ± 0.01NS
Tibials muscle0.081 ± 0.0030.076 ± 0.003NS
Subcutaneous fat0.31 ± 0.030.21 ± 0.020.0052
Gonadal fat0.33 ± 0.040.27 ± 0.03NS