Research Report
Copyright ©2014 Baishideng Publishing Group Inc.
World J Gastroenterol. Jul 7, 2014; 20(25): 8139-8150
Published online Jul 7, 2014. doi: 10.3748/wjg.v20.i25.8139
Table 1 Summary of data from long noncoding RNA microarray for three pairs of metastatic lymph nodes, normal lymph nodes and tumor tissue of colorectal cancer
Sample IDlncRNA
Fold change ≤ 4Fold change > 4TotalChanges lncRNA
R1MLN vs NLNUp-regulation7041618652087
Down-regulation10341881222
MLN vs tumorUp-regulation2864172045849052
Down-regulation242120474468
NLN vs tumorUp-regulation2915199949148895
Down-regulation230316783981
R2MLN vs NLNUp-regulation122335415773923
Down-regulation19344122346
MLN vs tumorUp-regulation4881055931109
Down-regulation45759516
NLN vs tumorUp-regulation212376328864514
Down-regulation12313971628
R3MLN vs NLNUp-regulation155739919563707
Down-regulation14413101751
MLM vs tumorUp-regulation91511410291572
Down-regulation48261543
NLN vs tumorUp-regulation148159720783619
Down-regulation11024391541
Table 2 Summary of data from message RNA microarray for three pairs of metastatic lymph nodes, normal lymph nodes and tumor tissue of colorectal cancer
SampleMessage RNA
IDFold change ≤ 4Fold change > 4TotalChanges lncRNA
R1Positive vs negativeUp-regulation142131017312818
Down-regulation9101771087
Positive vs tumorUp-regulation40282762679010525
Down-regulation211216233735
Negative vs tumorUp-regulation3848254263909875
Down-regulation208813973485
R2Positive vs negativeUp-regulation135454418984354
Down-regulation19604962456
Positive vs tumorUp-regulation5711297001534
Down-regulation711123834
Negative vs tumorUp-regulation180685826645103
Down-regulation16927472439
R3Positive vs negativeUp-regulation170661223184317
Down-regulation15624371999
Positive vs tumorUp-regulation86514210071855
Down-regulation731117848
Negative vs tumorUp-regulation132875620844569
Down-regulation16818042485
Table 3 Specifically expressed long noncoding RNAs in metastatic lymph nodes by comparing metastatic lymph nodes both with normal lymph nodes and tumor tissue of colorectal cancer
Up-regulated in MLNDown-regulated in MLN
LncRNA
Log2 fold change (M > N and M > T)
LncRNA
Log2 fold change (M < N and M < T)
uc004aej.22.466393114BC042589-4.323467267
ENST000003933112.086153236ENST00000429729-2.720521004
ENST000004475522.795028512uc002ztx.3-2.196481477
AK0251802.885255406ENST00000418346-2.260120093
AW4496733.034259412ENST00000465846-3.200848076
ENST000004505722.06748042
ENST000004831262.357461429
ENST000004257852.055384542
CR5997882.670107882
AK0977282.453511443
uc002jsc.12.854856902
BC0369146.696784453
AK0214447.763431269
AK3077963.345445171
Table 4 Verification of expression levels of differentially expressed long noncoding RNAs in metastatic lymph nodes by quantitative real-time polymerase chain reaction in 26 colorectal cancer patients with lymph node metastasis
AK307796/GAPDH
AK025180/GAPDH
AK021444/GAPDH
ENST00000465846/GAPDH
MLNNLNTumorMLNNLNTumorMLNNLNTumorMLNNLNTumor
R10.087600.034200.03580.07900.034800.031900.013700.004530.007350.027000.03470.06610
R20.066700.040900.04320.13400.077700.038700.009780.002250.005600.021200.04210.06440
R30.104170.045300.05240.09400.053000.045000.007620.003650.004350.020870.03340.05210
R40.047080.039900.05260.08000.060200.049380.010600.004150.005410.029000.02970.05370
R50.072000.032300.06080.09060.068600.054500.010300.003240.006080.029100.03900.06820
R60.079500.038500.06350.13100.060700.056900.011600.003630.007640.020150.02940.05900
R70.097100.033100.04010.08700.045200.035800.008130.002680.004550.021800.03030.05310
R80.056200.040240.06140.11100.071540.055440.007980.004270.007060.026800.03790.06635
R90.071100.034300.04140.08920.047500.037400.013200.002890.004950.022300.03150.06427
R100.061200.039100.05910.09230.046800.052330.008340.003080.007510.025200.03690.05480
R110.089800.042700.03980.12000.073130.039800.013400.004480.005080.023900.03250.06177
R120.062500.041330.06050.08270.070100.036700.007800.003970.005620.026380.03550.05610
R130.102500.035700.04230.10500.049900.050600.012700.003190.006830.023100.03390.05690
R140.058500.035920.05370.10700.051500.054700.013100.004080.006970.025560.03890.06105
R150.049810.034900.04490.09830.063600.040740.008830.003130.007190.024970.03310.05940
R160.096230.036800.06300.09620.050800.049800.008470.003750.004630.023500.03830.06370
R170.081200.040800.05070.10300.067500.042300.012100.003350.006520.020900.03050.06010
R180.078000.036230.06270.08390.048200.048500.012400.003670.004780.028600.03650.06505
R190.099100.038000.04620.11400.070840.044720.009320.003490.005310.020500.03110.05750
R200.084520.035240.05660.09980.054730.046340.011600.003810.007360.024750.03630.06810
R210.091100.042100.04370.08150.066800.043600.009560.003410.007590.024100.03180.05530
R220.067800.037940.05210.11600.061540.047400.008680.004030.005920.026000.03910.06287
R230.093800.043920.04750.12100.058230.045800.012500.002750.005780.024300.03290.05450
R240.067810.039000.05530.08810.060140.051430.009070.004410.006220.027300.03760.06590
R250.074300.041800.04910.11500.056800.041500.011900.002950.005170.022790.03090.05830
R260.053200.037530.05790.10200.068400.056300.012900.004210.006360.028130.03850.06668