Brief Article
Copyright ©2014 Baishideng Publishing Group Co.
World J Gastroenterol. Apr 28, 2014; 20(16): 4723-4729
Published online Apr 28, 2014. doi: 10.3748/wjg.v20.i16.4723
Table 1 Primers for polymerase chain reaction
PrimersSequencesAnnealing temp (°C)Size (bp)
MT1M
U5'-TTGAAAATGGTGGGGTGA-3'54163
5'-AAACTATACACCAAATAATACACAATATCC-3'
M5'-GACGTTCGCGACGTTAAG-3'54124
5'-ACGCCGAATAATACGCAAT-3'
MT1G
U5'-GGGGTTGTTTTGTGGTGTGTG-3'59135
5'-AAACACCCCACCCCACCCTT-3'
M5'-TTCGCGAGTCGGTGCGAAAG-3'5096
5'-CCGCGATCCCGACCTAAACT-3'
Table 2 Clinicopathological data and serum metallothionein 1M and metallothionein 1G promoter methylation in hepatocellular carcinoma patients
CharacteristicsnMT1M
MT1G
1M2UP value1M2UP value
Total number12159628536
Gender
Male10050500.55270300.896
Female21912156
Age (yr)
≥ 556732350.80746210.670
< 555427273915
HBV infection
Yes10050500.55269310.512
No21912165
Vascular invasion or metastasis
Yes4426180.08633110.387
No7733445225
Histological differentiation
Poor4118230.61026150.426
Moderate5027233812
Well301416219
TNM stage
I-II6426380.05844130.115
III-IV5733244123
Tumor multiplicity
Single7226360.74152200.565
Multiple4923263316
Tumor size(cm)
≥ 55938210.00138210.170
< 56221414715
Table 3 Correlation of metallothionein 1M and metallothionein 1G promoter methylation with tumor size
GeneTumor size (cm)
rsPvalue
MethylatedM (P25-P75)UnmethylatedM (P25-P75)
MT1M6.5 (4.0-9.0)3.9 (2.2-6.4)0.3210.000
MT1G4.4 (2.9-8.0)5.0 (3.5-7.9)-0.0490.590
Table 4 Vascular invasion or metastasis and a combination of metallothionein 1M and metallothionein 1G promoter methylation
CharacteristicnMT1M and MT1G
1M2UP value
Vascular invasion or metastasis
Yes4418260.018
No771661
Table 5 Sensitivity and specificity of gene sets for hepatitis B virus (+) hepatocellular carcinoma detection in chronic hepatitis B group
No.MarkerTP/FNFP/TNSensitivity (%)TP/(TP + FN)Specificity (%)TN/(TN + FP)
1AFP56/4414/2356.062.1
2MT1M50/502/3550.094.6
3MT1G69/316/3169.083.8
4MT1M/MT1G90/107/3090.081.1
Table 6 Sensitivity and specificity of gene sets for hepatocellular carcinoma detection in normal controls group
No.GeneTP/FNFP/TNSensitivity (%)TP/(TP+FN)Specificity (%)TN/(TN+FP)
1MT1M59/622/2948.893.5
2MT1G85/364/2770.287.1
3MT1M/MT1G110/115/2690.983.9