Exenatide improves hepatic steatosis by enhancing lipid use in adipose tissue in nondiabetic rats

doi:10.3748/wjg.v20.i10.2653

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Mar 14, 2014; 20(10): 2653-2663
Published online Mar 14, 2014. doi: 10.3748/wjg.v20.i10.2653

Table 1 Primer sequences

Gene	Forward	Reverse
SREBP1c	GGAGCCATGGATTGCACATT	AGGAAGGCTTCCAGAGAGGA
FAS	CTAGGTGGCTTTGGCCTGGA	CGAACGTGCTTGGCTTGGTA
ACC1	GTTCTGTTGGACAACGCCTTCA	GTCGCAGAAGCAGCCCATTAC
LPL	GTGACCAGGGACATGTGACTTTG	CTTGTACTTCGTTGTGGTGGGACTA
HSL	TTGCCTACTGCTGGGCTGTC	GACACGGTGATGCAGAGGTTC
ApoB	TAGCATGCTTGCTGACATAAATGGA	ATGGAGCTGCCGGAGGTAATC
CPT-1	CTGCCAGTTCCATTAAGCCACA	CAGCTATGCAGCCTTTGACTACCA
LCAD	AAGGCCTGCTTGGCATCAAC	CAGGGCCTGTGCAATTTGAGTA
ACOX1	GGCCGCTATGATGGAAATGTG	GGGCTTCAAGTGCTTGTGGTAA
Catalase	GAACATTGCCAACCACCTGAAAG	GTAGTCAGGGTGGACGTCAGTGAA
SOD2	GACTAGGCCACAGGGCATTCA	ACTCAGAAACCCGTTTGCCTCTAC
TNF	TGGCCCAGACCCTCACACTC	CTCCTGGTATGAAGTGGCAAATC
MCP1	TCACCAGCAGCAGGTGTCCCAAAGA	ACAGAAGTGCTTGAGGTGGTTGTGG
Mfn1	CCTTGTACATCGATTCCTGGGTTC	CCTGGGCTGCATTATCTGGTG
Mfn2	TCAGCCCGAGTACACCTACAGAGA	TGAGGGCCAAATGCAAGACA
Opa1	ATGCTCGCTATCACTGCCAAC	CGTTTGCCAGTAAGCAATTTAACC
Dnm1	ATGCCTGTGGGCTAATGAACAA	GTCTCGCGATACAGCGGAAG
GAPDH	GGCACAGTCAAGGCTGAGAATG	ATGGTGGTGAAGACGCCAGTA

SREBP1c: Sterol regulatory element-binding protein-1; FAS: Fatty acid synthase; ACC1: Acetyl-CoA carboxylase 1; LPL: Lipoprotein lipase; HSL: Hormone-sensitive lipase; ApoB: Apolipoprotein B; CPT1: Carnitine palmitoyltransferase-1; LCAD: Long-chain acyl-CoA dehydrogenase; ACOX1: Acyl-CoA oxidase-1; SOD2: Superoxide dismutase 2; TNF: Tumor necrosis factor; MCP1: Monocyte chemotactic protein-1; Mfn1: Mitofusin 1; Mfn2: Mitofusin 2; Opa1: Optic atrophy-1; Dnm1: Dynamin-1; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase.

Citation: Tanaka K, Masaki Y, Tanaka M, Miyazaki M, Enjoji M, Nakamuta M, Kato M, Nomura M, Inoguchi T, Kotoh K, Takayanagi R. Exenatide improves hepatic steatosis by enhancing lipid use in adipose tissue in nondiabetic rats. World J Gastroenterol 2014; 20(10): 2653-2663
URL: https://www.wjgnet.com/1007-9327/full/v20/i10/2653.htm
DOI: https://dx.doi.org/10.3748/wjg.v20.i10.2653