Anti-miRNA-221 sensitizes human colorectal carcinoma cells to radiation by upregulating PTEN

Table 1 Impact of miR-221 expression on Caco2 cell radiosensitivity

Group	D0	Dq	SF4	SER
Blank control + irradiation	1.681	5.630	0.5966
Negative control + irradiation	1.666	5.813	0.5858	1.009
Pre-miR-221 + irradiation	2.208	7.828	0.7453	0.761
Anti-miR-221 + irradiation	1.068	4.655	0.2984	1.574

Caco2 cells were transfected with negative control, pre-miR-221 or anti-miR-221 oligonucleotides. D₀ and D_q were determined by standardized software, and the sensitization enhancement ratio (SER) was calculated by determining the ratio of the D₀ of the control group vs treated cells. SF₄: Surviving fraction at 4 Gy.

Table 2 Impact of PTEN on miR-221-mediated Caco2 cell radiosensitivity

Group	D0	Dq	SF4	SER
Blank control + irradiation	1.677	5.590	0.5667
Anti-PTEN-siRNA + irradiation	2.327	7.788	0.7569	0.718
Anti-miR-221 + irradiation	1.082	4.702	0.2977	1.550
Anti-miR-221 and anti-PTEN-siRNA + irradiation	1.511	5.010	0.4788	1.110

Caco2 cells were pretreated with anti-PTEN-siRNA for 24 h prior to the addition of anti-miR-221. D₀ and D_q were determined by standardized software, and the sensitization enhancement ratio (SER) was calculated by determining the ratio of the D₀ of the control group vs treated cells. SF₄: Surviving fraction at 4 Gy.