siRNA-targeted inhibition of growth hormone receptor in human colon cancer SW480 cells

doi:10.3748/wjg.v19.i44.8108

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 19, Issue 44

This Article

Academic Content and Language Evaluation of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (4397)

All Articles published online

The chart showing PDF series, HTML series, Tables (1-3) series.

Item

Count

PDF

307

HTML

2556

Tables (1-3)

119

Sum=2982

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

781

Download

628

Sum=1409

Nov 28, 2013 (publication date) through Apr 27, 2024

Times Cited of This Article

Times Cited (5)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Brief Article

World J Gastroenterol. Nov 28, 2013; 19(44): 8108-8113
Published online Nov 28, 2013. doi: 10.3748/wjg.v19.i44.8108

Table 1 Weight changes of tumor-bearing mice (n = 6; mean ± SD)

	Weight (g, mean ± SD)
Time	NS	G₂	GH	FU	FU+G₂	G₂+FU+GH
Pre-operation	20.69 ± 0.67	21.92 ± 0.70	20.67 ± 0.57	21.93 ± 0.58	21.86 ± 0.73	21.25 ± 0.79
Inoculation 2 d	20.64 ± 0.60	21.86 ± 0.79	20.82 ± 0.56	20.89 ± 0.66¹	20.09 ± 0.55¹³	20.41 ± 0.85¹³
Inoculation 5 d	20.65 ± 0.49	21.56 ± 0.81	20.96 ± 0.54¹	20.94 ± 0.67¹	19.92 ± 0.58¹³⁵	20.42 ± 0.76¹
Inoculation 8 d	20.65 ± 0.65	21.53 ± 0.56¹	21.18 ± 0.44¹	20.41 ± 0.73¹³	19.92 ± 0.52¹²³⁵	20.53 ± 0.70¹³
Inoculation 11 d	20.67 ± 0.63	21.53 ± 0.73¹	21.27 ± 0.53¹⁴	19.86 ± 0.57¹³	20.06 ± 0.52¹²³⁵	20.63 ± 0.81¹⁵
Inoculation 14 d	20.61 ± 0.62	21.60 ± 0.68¹²	21.51 ± 0.44¹⁴	9.46 ± 0.52¹²³	19.86 ± 0.92¹²	20.86 ± 0.72¹⁴
Inoculation 17 d	20.68 ± 0.66	21.60 ± 0.71¹	21.64 ± 0.45¹²⁴	18.94 ± 0.47¹²³	19.40 ± 0.60¹²³	21.04 ± 0.78¹⁴

¹Compared with pre-operation, P < 0.05;

²Compared with NS group, P < 0.05;

³Compared with G₂ group, P < 0.05;

⁴Compared with FU group, P < 0.05;

⁵Compared with GH group, P < 0.05. NS: Normal saline; G₂: Recombinant plasmid; GH: Growth hormone; FU: 5-fluorouracil.

Table 2 Tumor volume changes in tumor-bearing mice (n = 6, mean ± SD)

	Subcutaneous tumor volume (mm³, mean ± SD)
Time	NS	G₂	GH	FU	FU+G₂	G₂+FU+GH
Inoculation 5 d	7.72 ± 1.61	7.93 ± 1.74	8.11 ± 1.65	7.42 ± 1.51	6.51 ± 1.20	7.33 ± 1.32
Inoculation 8 d	20.19 ± 4.91²³	13.44 ± 4.12¹³	33.28 ± 3.24¹²³	17.51 ± 5.75³	15.12 ± 5.01³	15.44 ± 4.23
Inoculation 11 d	106.02 ± 6.61²³	21.12 ± 4.04¹³	151.90 ± 8.31¹²³	21.00 ± 5.07¹³	19.22 ± 4.33¹³	22.97 ± 4.95¹²³
Inoculation 14 d	133.41 ± 6.43²³	20.00 ± 4.75¹³	178.93 ± 3.11¹²³	16.23 ± 6.51¹³	11.55 ± 4.11¹²³	12.12 ± 3.11¹²³
Inoculation 17 d	116.81 ± 0.61²³	9.71 ± 3.82¹³	149.01 ± 3.02¹²³	11.54 ± 2.42¹³	11.42 ± 1.11¹³	10.47 ± 1.02¹³

¹Compared with NS group, P < 0.05;

²Compared with G₂ group, P < 0.05;

³Compared with GH group, P < 0.05. NS: Normal saline; G₂: Recombinant plasmid; GH: Growth hormone; FU: 5-fluorouracil.

Table 3 △CT value of growth hormone receptor mRNA expression detected by real-time reverse transcription-polymerase chain reaction in all groups

	NS	LP	Negative	GH	G₂	FU	FU+G₂	G₂+FU+GH
△CT	12.63 ± 0.14	12.63 ± 0.43	12.67 ± 0.21	12.71 ± 0.39	14.12 ± 0.10¹	15.15 ± 0.44¹	16.46 ± 0.27¹²	15.37 ± 0.57¹²

¹Compared with NS group, P < 0.05;

²Compared with G₂ group, P < 0.05. NS: Normal saline; G₂: Recombinant plasmid; GH: Growth hormone; FU: 5-fluorouracil.

Citation: Zhou D, Yang J, Huang WD, Wang J, Zhang Q. siRNA-targeted inhibition of growth hormone receptor in human colon cancer SW480 cells. World J Gastroenterol 2013; 19(44): 8108-8113
URL: https://www.wjgnet.com/1007-9327/full/v19/i44/8108.htm
DOI: https://dx.doi.org/10.3748/wjg.v19.i44.8108