Investigation of genome instability in patients with non-alcoholic steatohepatitis

Table 1 Demographic and laboratory parameters of the patient and control groups

Parameter	n	mean	SD	P
Age (yr)				0.977
Patient	25	41.92	13.33
Control	25	41.80	13.09
BMI (kg/m2)				0.001
Patient	25	31.19	4.62
Control	25	25.07	4.14
HOMA-IR				0.001
Patient	25	6.71	4.68
Control	25	1.40	0.53
Hb (g/dL)				0.80
Patient	25	14.73	1.49
Control	25	14.64	1.30
Creatinine (mg/dL)				0.12
Patient	25	0.74	0.15
Control	25	0.80	0.13
AST (U/L)				0.001
Patient	25	56.08	29.11
Control	25	16.88	3.33
ALT (U/L)				0.001
Patient	25	92.20	41.43
Control	25	15.88	5.88
TG (mg/dL)				0.001
Patient	25	219.21	141.68
Control	25	102.56	57.98
HDL (mg/dL)				0.52
Patient	25	46.37	9.65
Control	25	48.72	15.31
LDL (mg/dL)				0.02
Patient	25	136.75	30.14
Control	25	114.63	34.13

BMI: Body mass index; HOMA-IR: Homeostasis model assessment insulin resistance; ALT: Alanine aminotransferase; AST: Aspartate aminotransferase; TG: Triglyceride; HDL: High density lipoprotein; LDL: Low density lipoprotein; Hb: Hemoglobin.

Table 2 Total numbers and frequencies of binucleated cells with micronucleus, nucleoplasmic bridges and nuclear buds scored using the cytokinesis-blocked micronucleus cytome assay in phytohemagglutinin-stimulated lymphocytes from patients with non-alcoholic steatohepatitis and the control subjects

ID	Age (yr)	Sex	No. of MN in BN cells1	Distribution of BN cells with					No. of BN cells with NPBs	No. of BN cells with NBUDs
				1MN	2MN	3MN	4MN	5MN
Patients with non-alcoholic steatohepatitis
1	45	M	19	15	-	-	1	-	25	19
2	60	M	24	19	1	1	-	-	46	11
3	50	M	33	24	1	1	2	-	33	22
4	66	M	41	39	1	-	-	-	20	22
5	22	M	8	8	-	-	-	-	32	17
6	43	M	33	29	2	-	-	-	25	14
7	36	F	14	14	-	-	-	-	21	17
8	51	F	9	9	-	-	-	-	16	10
9	35	M	21	16	-	-	-	1	25	14
10	42	M	11	11	-	-	-	-	12	15
11	30	F	28	24	2	-	-	-	50	20
12	22	M	11	7	2	-	-	-	17	15
13	31	M	12	10	1	-	-	-	21	16
14	41	M	15	13	1	-	-	-	18	25
15	33	M	10	10	-	-	-	-	30	10
16	44	F	22	17	1	1	-	-	58	21
17	47	M	20	13	2	1	-	-	40	17
18	20	M	25	16	1	1	-	1	14	10
19	22	M	24	20	-	-	1	-	32	8
20	46	F	37	27	2	2	-	-	25	10
21	68	F	36	26	5	-	-	-	63	28
22	48	M	17	15	1	-	-	-	28	6
23	60	M	22	18	-	-	1	-	36	19
24	45	F	24	20	2	-	-	-	25	14
25	41	M	24	19	1	1	-	-	20	10
The control subjects
1	45	M	5	5	-	-	-		11	3
2	60	M	8	6	1	-	-		10	5
3	50	M	7	7	-	-	-		6	5
4	66	M	11	9	1	-	-		12	6
5	22	M	2	2	-	-	-		2	4
6	43	M	6	4	1	-	-		1	4
7	36	F	3	3	-	-	-		9	4
8	51	F	9	5	2	-	-		5	3
9	35	M	4	2	1	-	-		4	1
10	42	M	6	6	-	-	-		7	5
11	30	F	8	8	-	-	-		1	4
12	22	M	2	2	-	-	-		8	4
13	31	M	2	2	-	-	-		10	5
14	41	M	7	5	1	-	-		16	8
15	33	M	7	7	-	-	-		12	6
16	44	F	11	8	-	1	-		5	6
17	47	M	9	7	1	-	-		4	2
18	20	M	1	1	-	-	-		8	4
19	22	M	4	4	-	-	-		9	1
20	46	F	11	7	2	-	-		8	3
21	65	F	17	15	1	-	-		9	5
22	48	M	12	10	1	-	-		14	6
23	60	M	6	6	-	-	-		13	3
24	45	F	11	9	1	-	-		5	3
25	41	M	3	3	-	-	-		7	5

The numbers of micronucleus (MN), nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) were scored on 1000 binucleated (BN) cells per subject.

¹Total number of MN: (1MNX1) + (2MNX2) + (3MNX3) + (4MNX4) + (5MNX5). M: Male; F: Female.

Table 3 The numbers of micronucleus, nucleoplasmic bridges and nuclear buds in phytohemagglutinin-stimulated lymphocytes from patients and controls (means ± SD)

Group	Age (yr)	No. of MN in BN cells	No. of BN cells with NPBs	No. of BN cells with NBUDs
Patients (n = 25)	41.92 ± 13.33	21.60 ± 9.32	29.28 ± 13.31	15.60 ± 5.55
Controls (n = 25)	41.80 ± 13.09	6.88 ± 3.91	7.84 ± 3.96	4.20 ± 1.63
P value	0.977	< 0.00011	< 0.00011	< 0.00011
Z value	0.029	5.413	5.953	6.016

The numbers of micronucleus (MN), nucleoplasmic bridges (NPBs) and nuclear buds (NBUDs) were scored on 1000 binucleated (BN) cells per subject.

¹Patients with non-alcoholic steatohepatitis exhibited statistically higher numbers of MN, NPBs and NBUDs in 1000 BN cells than controls, according to the two-tailed nonparametric Mann-Whitney U-test for the comparison of the means of independent variables.