Original Article
Copyright ©2013 Baishideng Publishing Group Co.
World J Gastroenterol. Jun 21, 2013; 19(23): 3562-3572
Published online Jun 21, 2013. doi: 10.3748/wjg.v19.i23.3562
Table 1 List of Enterococcus strains used in the experiment
Number of isolatesStrain numberSpecies (API 20 Strep/Multiplex PCR)Disease entity/Patient number
13AEnterococcus faeciumCD/1
23BEnterococcus faecium
310AEnterococcus aviumCD/2
412A/1Enterococcus faecalis
512A/2Enterococcus aviumCD/3
612BEnterococcus faecium
725AEnterococcus faeciumCD/4
825BEnterococcus avium
931BEnterococcus faecalisCD/5
1042A/1Enterococcus faecalisCD/6
1142A/2Enterococcus faecium
1251A/1Enterococcus faecalisCD/7
1351A/2Enterococcus avium
1457AEnterococcus faeciumCD/8
158AEnterococcus faecalisCD/9
168B/1Enterococcus faecalis
178B/2Enterococcus avium
1819BEnterococcus faeciumCD/10
1922BEnterococcus faecalisCD/11
2069AEnterococcus faecalisCD/12
2179AEnterococcus faecalisCD/13
2232BEnterococcus faecalisUC/1
2335AEnterococcus faecalisUC/2
2440AEnterococcus aviumUC/3
2540BEnterococcus avium
2648AEnterococcus faecalisUC/4
2758AEnterococcus faecalisUC/5
2877AEnterococcus faecalisUC/6
2933BEnterococcus aviumUC/7
3029BEnterococcus aviumUC/8
3134A/1Enterococcus faeciumUC/9
3234A/2Enterococcus avium
3364BEnterococcus faecalisUC/10
3465AEnterococcus faecalisUC/11
355BEnterococcus faecalisControl/1
367BEnterococcus faeciumControl/2
3715B/1Enterococcus aviumControl/3
3815B/2Enterococcus duransControl/4
3916B/1Enterococcus aviumControl/5
4016B/2Enterococcus durans
4127B/1Enterococcus faecalisControl/6
4227B/2Enterococcus faecium
4327B/3Enterococcus avium
4430B/1Enterococcus faeciumControl/7
4530B/2Enterococcus avium
4646BEnterococcus faecalisControl/8
4755BEnterococcus faecalisControl/9
4866BEnterococcus faecalisControl/10
Table 2 Presence of the gelE gene, quorum sensing genes (fsrA, fsrB, fsrC) and gelatinase activity in tested Enterococcus strains
SourceStrainsDetection of genes
Gelatinase activity on 16% gelatin plates
gelEfsrAfsrBfsrC
Crohn's diseaseEnterococcus faecalis 12A/1+--+-
Enterococcus faecalis 31B+-++-
Enterococcus faecalis 51A/1+--+-
Enterococcus faecium 3A+++++
Enterococcus faecium 57A+----
Enterococcus faecium 19B++---
Enterococcus avium 51A/2-----
Enterococcus avium 25B+--+-
Enterococcus avium 10A+----
Ulcerative colitisEnterococcus faecalis 35A+----
Enterococcus faecalis 48A+--+-
Enterococcus faecalis 77A+--+-
Enterococcus faecalis 32B+++++
Enterococcus avium 40A+----
Enterococcus avium 34A/2-----
Enterococcus avium 40B+----
ControlEnterococcus faecalis 5B+--+
Enterococcus faecalis 27B/1+++++
Enterococcus faecalis 46B+-+--
Enterococcus faecalis 55B-----
Enterococcus faecalis 66B-----
Enterococcus faecium 27B/2+----
Enterococcus faecium 30B-----
Enterococcus avium 15B/1-----
Enterococcus avium 16B/1-----
Enterococcus avium 27B/3+----
Enterococcus durans 15B/2-----
Enterococcus durans 16B/2-----
ATCC controlEnterococcus faecalis 29212+--+-
Enterococcus faecium 35667-----
Table 3 Presence of asa1 gene, degree of adhesion to human gut epithelium cells and biofilm production among Enterococcus strains
SourceStrainsasa1AdherenceBiofilm production (after 48 h) A492 nm± SD
Crohn's diseaseEnterococcus faecalis 12A/1-+++0.995 ± 0.351
Enterococcus faecalis 31B-+++1.302 ± 0.585
Enterococcus faecalis 51A/1-++1.083 ± 0.413
Enterococcus faecium 3A--1.286 ± 0.088
Enterococcus faecium 57A++++0.698 ± 0.066
Enterococcus faecium 19B++++1.087 ± 0.368
Enterococcus avium 51A/2--2.101 ± 0.424
Enterococcus avium 25B--2.474 ± 0.683
Enterococcus avium 10A--1.423 ± 0.284
Ulcerative colitisEnterococcus faecalis 35A++++1.528 ± 0.326
Enterococcus faecalis 48A-+++0.767 ± 0.582
Enterococcus faecalis 77A-++0.822 ± 0.196
Enterococcus faecalis 32B+++0.557 ± 0.344
Enterococcus avium 40A-+++0.859 ± 0.152
Enterococcus avium 34A/2--
Enterococcus avium 40B--0.282 ± 0.078
ControlEnterococcus faecalis 5B-0.512 ± 0.065
Enterococcus faecalis 27B/1--0.934 ± 0.103
Enterococcus faecalis 46B-++0.507 ± 0.095
Enterococcus faecalis 55B--0.408 ± 0.242
Enterococcus faecalis 66B--0.172 ± 0.031
Enterococcus faecium 27B/2-0.917 ± 0.269
Enterococcus faecium 30B--0.359 ± 0.087
Enterococcus avium 15B/1--
Enterococcus avium 16B/1--0.305 ± 0.104
Enterococcus avium 27B/3--0.657 ± 0.182
Enterococcus durans 15B/2--
Enterococcus durans 16B/2--0.315 ± 0.246
ATCC controlEnterococcus faecalis 29212++++0.877 ± 0.221
Enterococcus faecium 35667-++0.722 ± 0.371
Table 4 Mean amounts of hydrogen peroxide (in mmol/L) produced in vitro after 4 and 24 h by the tested strains
SourceStrainsH2O2 production (mmol/L) after 4 hH2O2 production (mmol/L) after 24 h
Crohn's diseaseEnterococcus faecalis 12A/100
Enterococcus faecalis 31B00
Enterococcus faecalis 51A/100
Enterococcus faecium 3A00
Enterococcus faecium 57A00
Enterococcus faecium 19B00.3
Enterococcus avium 51A/200.3
Enterococcus avium 25B00.3
Enterococcus avium 10A0.31
Ulcerative colitisEnterococcus faecalis 35A00
Enterococcus faecalis 48A00
Enterococcus faecalis 77A00
Enterococcus faecalis 32B00
Enterococcus avium 40A00
Enterococcus avium 34A/200.3
Enterococcus avium 40B00
ControlEnterococcus faecalis 5B00
Enterococcus faecalis 27B/100
Enterococcus faecalis 46B00
Enterococcus faecalis 55B00
Enterococcus faecalis 66B00
Enterococcus faecium 27B/200.3
Enterococcus faecium 30B00
Enterococcus avium 15B/10.31
Enterococcus avium 16B/100
Enterococcus avium 27B/300.3
Enterococcus durans 15B/200
Enterococcus durans 16B/200