Oncogene GAEC1 regulates CAPN10 expression which predicts survival in esophageal squamous cell carcinoma

Table 1 List of more than 5-fold and 3-fold down-regulated genes induced by stable GAEC1 knowndown in KYSE150 cells compared with the parental cells

Probe set ID	Gene title	Down-regulation with transfected pSilencer P3-4	Up-regulation with transfected pcDNA3.1-GAEC1	pSilencer-ve control
221040_at	Calpain 10	15.3033010	2.1643467	1.0476209
1561417_x_at	Not assigned	12.1628650	2.0130675	1.1347373
1562828_at	Not assigned	9.9816000	2.6808436	1.1661105
229929_at	splA/ryanodine receptor domain and SOCS box containing 4	8.3420770	2.2365010	1.1751518
235209_at	Chromosome 8 open reading frame 84	7.6294910	2.1356385	1.1450081
220090_at	Cornulin	7.6125007	2.3401918	1.1664450
242713_at	Not assigned	7.3507795	2.0578532	1.1959343
224499_s_at	Activation-induced cytidine deaminase	5.8917794	3.3146940	1.1664389
229543_at	Not assigned	5.3493247	2.0206234	1.0065930
242064_at	Sidekick homolog 2 (chicken)	5.0322995	2.8469403	1.0170712
1561041_at	Trinucleotide repeat containing 6C	7.5979643	2.1234870	1.0152589
216787_at	Not assigned	5.3369575	2.2657390	1.1658608
206725_x_at	Bone morphogenetic protein 1	4.8828310	2.7046654	1.1015952
206276_at	Lymphocyte antigen 6 complex, locus D	4.7652740	2.7379642	1.0686288
1560482_at	Not assigned	4.2348604	3.0122058	1.1875614
211362_s_at	Serpin peptidase inhibitor, clade B (ovalbumin), member 13	4.0584164	2.4208739	1.0186443
216491_x_at	Immunoglobulin heavy constant mu	3.4819565	2.8060850	1.0186309
238415_at	Not assigned	3.2034543	2.9523630	1.0138865
241028_at	RPGRIP1-like	3.0331728	2.3752263	1.0001514

All the listed genes were selected based on more than 2-fold of the corresponding up-regulation when GAEC1 was overexpressed with transfected pcDNA3.1-GAEC1 vector and no significant fold change with transfected pSilencer-negative control vector compared with parental cells.

Table 2 Relationship between CAPN10 expression and clinicopathological features

Characteristics	Patients	Low expression	High expression	P value
Age, yr (mean ± SD)	132	65.64 ± 10.55	63.50 ± 13.39	0.572
Gender				0.086
Male	102	94	8
Female	30	24	6
TNM stage				0.762
0/I/II	18	15	9
III/IV	83	72	11
Tumor depth				0.729
T1-3	79	67	12
T4	22	20	2
Lymph node metastasis				0.363
N0	32	26	6
N1	69	61	8
Distant metastasis				1
M0	66	57	9
M1	35	30	5
Differentiation				0.459
Well	16	15	1
Moderate	59	51	8
Poor	26	21	5