Immunohistochemical study of the digestive tract of Oligosarcus hepsetus

Table 1 Details of primary antisera used in this study

Primary antisera	Donor	Code No.	Working dilution	Source
Somatostatin	Rabbit	A566	1:300	Dako Corp., CA, United States
Serotonin	Rabbit	S5545	1:8.000	Sigma-Aldrich, Inc., United States
Cholecystokinin	Rabbit	C2581	1:8.000	Sigma-Aldrich, Inc., United States
Gastrin	Rabbit	G0785	1:1.000	Sigma-Aldrich, Inc., United States
Glucagon	Mouse	G2654	1:2.000	Sigma-Aldrich, Inc., United States
Insulin	Mouse	I2018	1:1.000	Sigma-Aldrich, Inc., United States

Table 2 Regional distribution and intensity of immunoreaction in endocrine cells in the digestive tract of Oligosarcus hepsetus

Antisera	Segment of the esophagus	Segments of the stomach		Segments of the gut
		Gl	NGl	ANT	POST
Somatostatin	+++	++	+	-	-
Serotonin	-	+++	++	-	-
Cholecystokinin	-	-	-	-	++
Glucagon	-	-	-	-	-
Gastrin	-	-	-	-	-
Insulin	-	-	-	-	-

Intesity of immunoreactions: (-), absent; (+), low; (++), medium; (+++), strong. Gl: Glandular; NGl: Non-glandular; ANT: Anterior; POST: Posterior.