Epigenetic inactivation of secreted frizzled-related protein 2 in esophageal squamous cell carcinoma

Table 1 List of primer sequences

Primer	Forward primer (5'-3')	Reverse primer(5'-3')
RT-PCR
SFRP2	5’-GATGATGACAACGACATAATGGAAACG -3’	5’-GAGTGTGCTTGGGGAACGGGAGCT-3’
GAPDH	5’-CCCTTCATTGACCTCAACTACATGG-3’	5’-CATGGTGGTGAAGACGCCAG-3’
SFRP2(CDS)	5’-CCAAGCTTATGCTGCAGGGCCCTGGCTCGC-3’	5’-CGGAATTCCTAGCACTGCAGCTTGCGGATGCTG-3’
MSP
SFRP2-M	5’-GGGTCGGAGTTTTTCGGAGTTGCGC-3’	5’-CCGCTCTCTTCGCTAAATACGACTCG-3’
SFRP2-U	5’-TTTTGGGTTGGAGTTTTTTGGAGTTGTGT-3’	5’-AACCCACTCTCTTCACTAAATACAACTCA-3’
BSP
SFRP2	5’-AAAAAGGTTAAGAAAATTTTGGT-3’	5’-AACCAAAACCCTACAACATC-3’

SFRP2: Secreted frizzled-related protein 2; GAPDH: Glyceraldehyde-3-phosohate dehydrogenase; MSP: Methylation-specific.

Table 2 Effect of secreted frizzled-related protein 2 re-expression on the cell cycle distribution of KYSE30 cells (%)

Groups	G0/G1	S	G2/M
KYSE-30/vector	45.76 ± 3.74	48.64 ± 4.90	5.59 ± 5.18
KYSE-30/SFRP2	64.49 ± 6.11	30.52 ± 5.07	4.97 ± 6.60