Identification of deregulated miRNAs and their targets in hepatitis B virus-associated hepatocellular carcinoma

Table 1 Characteristics of patients (n = 18)

No.	Gender	Age (yr)	Tumor size1 (cm)	Cirrhosis	Tumor grade2	TNM stage
1	M	55	1.9	No	G3	T2N0M0
2	M	40	4.1	Macronodular	G3	T2N0M0
3	M	42	4.6	No	G3	T3N0M0
4	M	42	5.6	Micronodular	G2	T3N0M0
5	M	43	5.8	Micronodular	G1	T1N0M0
6	M	61	1.9	Micronodular	G3	T2N0M0
7	M	55	12.0	Micronodular	G2	T4N0M0
8	M	40	4.0	Macronodular	G2	T3N0M0
9	M	35	2.3	Macronodular	G1	T1N0M0
10	M	57	5.9	No	G3	T1N0M0
11	M	44	9.6	No	G2	T3N0M0
12	M	45	12.2	Micronodular	G3	T2N0M0
13	M	46	4.4	Micronodular	G3	T2N0M0
14	F	46	15.8	Micronodular	G3	T2N0M0
15	M	63	6.1	No	G3	T3N0M0
16	M	61	4.6	No	G3	T2N0M0
17	F	60	9.1	No	G3	T3N0M0
18	F	50	9.3	No	G3	T2N0M0

¹Diameter of the biggest nodule.

²G1-2: Well-differentiated; G3: Moderately-differentiated; G4: Poorly-differentiated. M: Male; F: Female; TNM: Tumor-node-metastasis.

Table 2 Deregulated miRNA in hepatitis B virus-associated hepatocellular carcinoma n (%)

miRNA	Fold change	P value	Validation1
Up-regulated miRNAs
hsa-miR-520g	9475.09	0.00	13 (72.2)
hsa-miR-519a	8204.48	0.00	10 (55.6)
hsa-miR-522	6178.34	0.00	13 (72.2)
hsa-miR-518e	4096.74	0.00	11 (61.1)
hsa-miR-525-3p	1489.49	0.00	14 (77.8)
hsa-miR-217	1080.33	0.00	11 (59.4)
hsa-miR-518b	982.85	0.00	14 (77.8)
hsa-miR-512-3p	197.21	0.00	10 (55.6)
hsa-miR-517c	130.92	0.00	11 (61.1)
hsa-miR-518a-3p	46.84	0.00	10 (55.6)
Down-regulated miRNAs
hsa-miR-199a-5p	0.00237	0.00050	13 (72.2)
hsa-miR-433	0.00310	0.00270	10 (55.6)
hsa-miR-592	0.01137	0.00350	11 (59.4)
hsa-miR-214#	0.01327	0.00610	11 (59.4)
hsa-miR-483-5p	0.03310	0.00340	14 (77.8)
hsa-miR-483-3p	0.03323	0.00510	12 (72.2)
hsa-miR-138	0.03727	0.00190	14 (77.8)
hsa-miR-214	0.04213	0.00530	11 (59.4)
hsa-miR-511	0.07957	0.00490	13 (72.2)
hsa-miR-708	0.02135	0.00480	10 (55.6)
hsa-miR-1275	0.06296	0.00530	10 (55.6)

¹Quantitative reverse-transcription polymerase chain reaction was performed in 18 pairs of tumor and non-tumor tissues in hepatitis B virus-associated hepatocellular carcinoma.