Copyright ©2012 Baishideng Publishing Group Co.
World J Gastroenterol. Aug 14, 2012; 18(30): 3923-3930
Published online Aug 14, 2012. doi: 10.3748/wjg.v18.i30.3923
Table 1 Osteopontin as a potential therapeutic target for gastric and liver cancers
Cell linesMouse modelMethod of studyResultant effectsPossible mechanisms
Gastric cancer
SGC7901Nude micesiRNA knockdownReduced angiogenesis in vitro and in vivoDecreasing microvessel density
BGC-823Nude miceTransient/stable siRNA knockdownInhibited cell growth, anchorage-independent growth, migration and invasion in vitro, and suppressed tumor growth and prolonged survival in vivoInhibition of MMP-2 and uPA expression, NF-κB DNA binding activity, and Akt phosphorylation
SGC7901Nude mice implanted with SGC-OPN-cellsLentivirus-mediated stable depletionSuppressed metastases and prolonged survival time in vivoReducing expression of VEGF
MHCC97-L, MHCC97-H, HCC-LM3siRNA knockdownDecreased cell invasion and cell cloning number in vitro
HuH1/4/7, MHCC97, SMMC7721, SK-Hep-1, Hep3B, CCL13, HCCLM3Nude mice of lung metastasisOPN-neutralizing antibodyBlocked invasion of SK-Hep-1 and Hep3B cells in vitro, inhibited pulmonary metastasis of HCC-LM3 cells in vivo
HCC-LM6Nude mice implanted with HCC-LM6Antisense knockdownSuppressed migration and invasion in vitro, decreased lung metastases in vivoInhibiting MMP-2 and uPA expression
HCC-LM3Nude mice implanted with Lenti OPNi- transfected HCC-LM3 cellsStable depletion using lentiviral vectors encoding miRNA against OPNInhibited both in vitro proliferation, invasion and in vivo tumor growth and lung metastasisInhibiting MAPK and NF-κB pathways, and MMP-2 and suppressing uPA expression
HCC-LM3 HepG2Nude miceshRNA gene silencingInhibited HCC cell growth, adhesion and invasion in vitro, and suppressed tumorigenicity and lung metastasis in vivo, enhanced sensitivity of HCC cells to chemotherapeutic drugsSuppressing αv, β1, β3 integrin expression, blocking NF-κB activation, inhibiting apoptosis