Late SV40 factor: A key mediator of Notch signaling in human hepatocarcinogenesis

Table 1 Reverse transcription-polymerase chain reaction primer sequences

Primer	Sequence (5’-3’)	Product size (bp)	Temperature (°C)
Notch1-ICD	Forward: CTAAGATCTCCTGAGGGCTTCAAAGTGTC	2375	63
	Reverse: GCGAATTCCTTGAAGGCCTCCGGAAT
H-Ras (ORF)	Forward: GAGGATCCATGACGGAATATAAGCTGG	668	61
	Reverse: GTGAATTCTCAGGAGAGCACACACTTG
Hes-1	Forward: ATGAACGAGGTGACCCGCTT	443	62
	Reverse: CTGGAAGGTGACACTGCGTT
LSF (ORF)	Forward: GGAAAGATCTAGGATGGCCTGGGCTCTGAAG	1547	59
	Reverse: CAAAGCTTGGGCACGAAACGCCGCACTCCT
LSF (promoter analysis)	Forward: CTAGGTACCCAACATGGTAAGATCCTGTCTCT	2020	56
	Reverse: GGAGATCTTCTCATCCCTGCTTTCTGTTTCCT
GAPDH	Forward: GGTGGAGGTCGGAGTCAACGGA	240	60
	Reverse: GAGGGATCTCGCTCCTGGAGGA

ORF: Open-reading frame; ICD: Intracellular domain; LSF: Late SV40 factor; LSF (promoter analysis): Upstream sequence before start code.

Table 2 Association between the activation Notch-1 and late SV40 factor in liver cancer specimens and clinicopathological features

Patients	n	Notch1	LSF2	Notch1 and LSF3	P value
Gender					0.5621,1.0002, 0.3123
Male	20	16	17	14
Female	5	3	4	2
Age (yr)					0.1941, 0.5482, 0.0753
< 50	7	4	5	2
≥ 50	18	15	16	13
Histological type					0.0591, 0.0812, 0.3593
HCC	17	15	16	13
Bile duct cell carcinoma	1	0	0
Nodular hepatocellular carcinoma	6	4	5	4
Other	1	0	0
Cellular differentiation					0.1371, 0.2942, 1.0003
Well/moderately	18	12	14	11
Poor/undifferentiated	7	7	7	5
Stage					0.0061, 0.0062, 0.013
I/II	8	3	4	2
III/IV	17	16	17	14

¹Notch1 P value;

²LSF P value;

³Notch1 and LSF P values; Fisher’s exact test. HCC: Hepatocellular carcinoma; LSF: Late SV40 factor.