Intrahepatic natural killer T cell populations are increased in human hepatic steatosis

Table 1 Patient characteristics

	No. steatosis(n = 10)	Mild steatosis(n = 11)	Moderate-severe steatosis(n = 6)	Reference values
Age	36.3 ± 13.1	44.5 ± 14.4	48.3 ± 11.3	N/A
Gender (% female)	90%	73%	66%	N/A
Body mass index (kg/m2)	42.4 ± 3.2	44.2 ± 6.2	41.9 ± 4.3	18.5-24.9
Aspartate transaminase (U/L)	28.8 ± 13.2	42.8 ± 34.2	47.0 ± 26.9	0-40
Alanine transaminase (U/L)	36.1 ± 18.7	50.1 ± 48.2	75.8 ± 40.1	0-45
Alkaline phosphatase (IU/L)	79.2 ± 18.0	89.5 ± 16.6	82.5 ± 12.7	20-140

All results except gender presented as mean ± standard deviation.

Table 2 Percentage of CD3+ lymphocyte populations in patients with normal livers, mild hepatic steatosis, and moderate to severe steatosis n (%)

Lymphocyte population	N steatosis	M steatosis	MS steatosis	P value(NvsM)	P value(NvsMS)	P value(MvsMS)	Correlationcoefficient
CD3+/CD4-/CD8- PBMC	8.13	3.62	22.88	0.22	0.1	0.04a	0.73
CD3+/CD4-/CD8- IHL	12.61	9.12	26.58	0.4	0.1	0.05a	0.76
CD3+/CD56+ PBMC	5.09	2.45	12.32	0.22	0.049a	0.016a	0.71
CD3+/CD56+ IHL	21.49	24.13	38.62	0.7	0.03a	0.048a	0.93
CD3+/CD56+/CD161+ PBMC	2.45	1.15	9.64	0.3	0.027a	0.017a	0.79
CD3+/CD56+/CD161+ IHL	15.50	18.90	35.81	0.6	0.006a	0.017a	0.93
CD3+/Vα24+ PBMC	0.60	0.53	0.57	0.48	0.23	0.14	-0.43
CD3+/Vα24+ IHL	0.43	0.42	0.76	0.9	0.37	0.36	0.85
CD3+/CD8+ IHL	55.59	49.30	26.58	0.51	0.0003a	0.006a	-0.95

Each percentage is the proportion of a specific CD3+ lymphocyte population out of all CD3+ lymphocytes.

^aP < 0.05. PBMC: Peripheral blood mononuclear cell; IHL: Intrahepatic lymphocyte. N: Normal; M: Mild; MS: Mod/sev.