Alterations of tumor-related genes do not exactly match the histopathological grade in gastric adenocarcinomas

Table 1 Different pilot experiments and probe sequence of 15 tumor-related genes

Tumor-related genes	Probe sequence
APC	5-TTGGTTCCCAGATGACTTGTCAGCC(T)TCGAGGTGCAGAGTGTGTG CTACTAG-3dig
Bcl10	5-CTGTATCAGGAAGTTCTGTG(T)TTTTTCTCGCCGAATAG ATTCAACAAGGGTG-3dig
Beclin1	5-CCAAGCAGCATTAATCTCATTCCA(T)TCCACGGGAACACTGGGCAGGCGACC-3dig
BRCA1	5-CCTCTTTCTTCATCATCTGAAACCAAT(T)CCTTGTCACTCAGACCAACTCCCT-3dig
BRCA2	5-AAGCGATGATAAGGGCAGAGGAAAAGG(T)CTAGGGTC AGGAAAGAATCCAAGT-3dig
FHIT	5-AGTCCTCCTTGTCATGTTTCTGGAGC(T)CCTCATAGATGCTGT CATTCCTGTG-3dig
KAI1	5-GCAGAAGCCCTTCCTCACAGAAAGGC(T)GTTGTCCTCT TCCCCCTTGACTTCGC-3dig
NM23	5-GGAATCCTTTCTGCTCAAAACGC(T)TGATAATCTCTCCCACAAGACCCCGCTG-3dig
Ptch	5-CGCTTCTGTGGTCAGGACAT(T)AGCACCTTCTTCTTTAG GGGTCTGTATCAT-3dig
PTEN	5-CCTCTTGATATCTCCTTTTGTTTC(T)GCTAACGATCTCTTTGATGATGGCTG-3dig
RB	5-TGAGCACACGGTCGCTGTTACA(T)ACCATCTGATTTATTTTCTGGAA CTTCT-3dig
UVRAG	5-CTCCTTGTTCTTGGCTAGGGTGCACA(T)TCGCGTGGCCT CCGTTTAAGCTGCCAAC-3dig
TP53	5-CCAGGACAGGCACAAACACGCACCT*CAAAGCTGTTC CGTCCCAGTAGATTAC-3dig
Cyclin D1	5-CCTCCTCGCACTTCTGTTCCTCGCAGACCT*CCAGCATCCAGGTGGCGACGATCTTCCG-3dig
C-myc	5-CTTCCTCATCTTCTTGTTCCTCCTCAGAGT*CGCTGCTGGTGGTGGGCGGTGTC-3dig

Table 2 P values of 15 genes in different comparisons

APC	Bcl10	Beclin1	BRCA1	BRCA2	FHIT	KAI1	NM23	Ptch	PTEN	RB	UVRAG	TP53	Cyclin D1	C-myc
0.0523	0.0002	0.0000	0.0000	0.0000	0.0000	0.0000	0.2603	0.0000	0.0006	0.0000	0.5295	0.0000	0.0000	0.0048
0.0000	0.7527	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0000	0.0001
0.0000	0.0018	0.0001	0.0371	0.0028	0.1999	0.0129	0.0000	0.1142	0.0001	0.0009	0.0000	0.0092	0.3676	0.0158