Original Article
Copyright ©2010 Baishideng.
World J Gastroenterol. Jan 21, 2010; 16(3): 312-319
Published online Jan 21, 2010. doi: 10.3748/wjg.v16.i3.312
Table 1 Primer Sequences and PCR conditions for methylation-specific PCR (MSP) analysis
GenePrimer (5’-3’) forwardPrimer (5’-3’) reverseSize (bp)T (°C)Ref.
COX2M TTAGATACGGCGGCGGCGGCTCTTTACCCGAACGCTTCCG16168[24]
U ATAGATTAGATATGGTGGTGGTGGTCACAATCTTTACCCAAACACTTCCA17167
DAPKM GGATAGTCGGATCGAGTTAACGTCCCCTCCCAAACGCCGA9865[25]
U GGAGGATAGTTGGATTGAGTTAATGTTCAAATCCCTCCCAAACACCAA11665
CDH1M TTAGGTTAGAGGGTTATCGCGTTAACTAAAAATTCACCTACCGAC11561[26]
U TAATTTTAGGTTAGAGGGTTATTGTCACAACCAATCAACAACACA9759
hMLH1M TATATCGTTCGTAGTATTCGTGTTCCGACCCGAATAAACCCAA15365[26]
U TTTTGATGTAGATGTTTTATTAGGGTTGTACCACCTCATCATAACTACCCACA12463
CDKN2AM TTATTAGAGGGTGGGGCGGATCGCGACCCCGAACCGCGACCGTAA15070[21]
U TTATTAGAGGGTGGGGTGGATTGTCAACCCCAAACCACAACCATAA15170
Table 2 Clinicopathological and epidemiological features of patients in the four tumor stages n (%)
VariablesPatientsTumor stage
0-IIIIIIIV
Age (yr)
≤ 50193 (15.8)2 (10.5)9 (47.4)5 (26.3)
> 50708 (11.4)11 (15.7)30 (42.9)21 (30)
Gender
Male608 (13.3)8 (13.3)27 (45)17 (28.3)
Female293 (10.3)5 (17.2)12 (41.4)9 (31)
Anatomic subsite
Proximal (Cardia)131 (7.7)1 (7.7)5 (38.5)6 (46.1)
Distal (Antrum/body)462 (4.3)9 (19.6)21 (45.7)14 (30.4)
Mixed30 (0)1 (33.3)2 (66.7)0 (0)
Laurén type
Diffuse314 (12.9)4 (12.9)12 (38.7)11 (35.5)
Intestinal577 (12.3)9 (15.8)27 (47.4)14 (24.6)
Mixed10 (0)0 (0)0 (0)1 (100)
Table 3 Associations between gene methylation status and clinicopathological features, age, H. pylori, MSI and EBV n (%)
Methylated genes
TotalCDH1P-valueDAP-kinaseP-valueCOX2P-valuehMLH1P-valueCDKN2AP-value
Mean age (yr)60.259.960.963.162.5
Age group (yr)
≤ 501910 (52.6)0.8838 (44.4)0.2829 (50)0.1796 (33.3)0.9033 (15.8)0.0351
> 506935 (50.7)41 (58.6)45 (67.2)21 (31.8)29 (42)
Gender
Male5930 (50.8)0.93833 (55.9)0.94634 (59.6)0.28921 (35)0.78721 (35.6)0.830
Female2915 (51.7)16 (55.2)20 (71.4)11 (37.9)11 (37.9)
Nodal metastases
Absent178 (47.1)0.7089 (50)0.58611 (64.7)0.9106 (33.3)0.9036 (33.3)0.764
Present7137 (52.1)40 (57.1)43 (63.2)21 (31.8)26 (37.1)
Tumor site
Cardia138 (61.5)0.8559 (69.2)0.42710 (83.3)0.2284 (30.8)0.3048 (61.5)0.266
Antrum3821 (55.3)20 (52.6)21 (56.8)14 (37.8)14 (35.9)
Body105 (50)7 (70)7 (70)1 (11.1)4 (40)
Misted33 (100)2 (66.7)3 (100)00
Laurèn
Diffuse3017 (56.7)0.50310 (33.3)0.62819 (63.3)0.9738 (27.6)0.48110 (33.3)0.628
Intestinal5728 (49.1)22 (38.6)34 (63)19 (35.2)22 (38.6)
Mixed10 (0.0)01 (100)00
H. pylori
CagA+5531 (56.4)0.26130 (54.5)0.78235 (66)0.53615 (28.8)0.4122 (40)0.468
CagA-3415 (44.1)19 (57.6)19 (59.4)12 (37.5)11 (32.4)
MS status
MSI145 (35.7)0.1744 (26.7)0.01218 (53.3)0.34910 (66.7)0.00114 (26.7)0.405
MSS7240 (55.6)44 (62)45 (66.2)16 (23.5)27 (38)
EBV
Positive51 (20)0.1014 (80)0.3464 (80)0.69400.1793 (60)0.462
Negative4828 (58.3)28 (58.3)33 (71.7)15 (31.9)21 (42.9)