Hydrophobic protein in colorectal cancer in relation to tumor stages and grades

Table 1 Clinical information of the patients studied

No.	Age (yr)	Sex	Duke’s system	Localization	Degree of differentiation
1	57	F	C	Right colon	Well-differentiated
2	64	M	C	Caecum	Moderately-differentiated
3	45	M	B	Sigmoid colon	Well-differentiated
4	91	F	C	Sigmoid colon	Well-differentiated
7	75	F	B	Rectosigmoid	Well-differentiated
8	62	M	C	Rectum	Well-differentiated
9	71	F	C	Sigmoid colon	Moderately-differentiated
10	76	M	B	Rectosigmoid	Well-differentiated
12	70	M	C	Sigmoid colon	Moderately-differentiated
13	63	M	B	Rectum	Moderately-differentiated
14	62	F	B	Rectosigmoid	Well-differentiated
15	54	F	B	Descending colon	Well-differentiated
17	74	M	B	Rectosigmoid	Moderately-differentiated
19	67	M	B	Rectum	Moderately-differentiated
20	53	F	C	Rectosigmoid	Moderately-differentiated
21	79	F	C	Rectosigmoid	Moderately-differentiated
23	67	M	C	Sigmoid colon	Moderately-differentiated
24	42	M	B	Descending colon	Well-differentiated

Table 2 Differentially expressed proteins in CRC

Protein spot	Protein name	SwissProt accession No.	Score	Theoretical molecular weight (Da)	Theoretical pI	Sequence coverage (%)	GRAVY	Fold change1	TMR
S1	F1-ATPase β subunit	P06576	364	58 013	5.80	19	-0.030	+58.5	1
S2	Ubiquinol-cytochrome c reductase	P31930	171	53 308	5.94	7	-0.141	+32.8	1
S3	Calreticulin	P27797	73	47 092	4.30	11	-1.191	-31.9	1
S41f	Tubulin α-1 chain	Q71U36	61	50 800	4.94	6	-0.229	+10.8	1
S51cf	Tubulin β-2 chain	P68371	299	48 142	4.70	25	-0.347	+20.8	1
S6	Chaperonin GroEL	P10809	185	61 348	5.70	8	-0.076	+265.0	3
S7	Heat shock 70 kDa protein	P11021	775	72 488	5.07	42	-0.487	+5.1	1
S8abcdefg	Stomatin-like protein 2	Q9UJZ1	151	38 644	6.88	28	-0.161	+27.9	1
S9ae	Annexin A3	P12429	140	36 396	5.63	22	-0.430	+34.9	0
S10abcef	Annexin A4	P09525	165	35 983	5.85	33	-0.447	+29.6	0
S11	Prohibitin	P35232	421	29 890	5.57	41	+0.024	+11.8	1
S12	Annexin A5	P08758	195	35 994	4.94	39	-0.330	-5.2	0
S13	ATP Synthase D chain	O75947	117	18 406	5.22	32	-0.569	+8.3	0

¹Fold change is given as the ratio of the spot intensity in normal mucosa over tumour tissue (negative variation or decrease) or tumor tissue over normal tissue (positive variation or increase).

^aDifferentially expressed proteins that are statistically significant (P < 0.05) among all 18 patients;

^bDifferentially expressed proteins that are statistically significant (P < 0.05) in Duke’s B stage;

^cDifferentially expressed proteins that are statistically significant (P < 0.05) in Duke’s C stage;

^dDifferentially expressed proteins that are statistically significant (P < 0.05) in WDC;

^eDifferentially expressed proteins that are statistically significant (P < 0.05) in MDC;

^fDifferentially expressed proteins that are statistically significant (P < 0.05) in female patients;

^gDifferentially expressed proteins that are statistically significant (P < 0.05) in male patients. pI: Isoelectric point; GRAVY: Grand average of hydrophobicity; TMR: Transmembrane region.