Omentum facilitates liver regeneration

Table 1 Primer sequences of the selected developmental genes that were tested in the regenerating liver tissue by RT-PCR technique

Gene1	Primer	Predicted size (bp)	Accession number2	Entrez gene ID2
β-actin	F (926) 5'-TCATGAAGTGTGACGTTGACATCCGT-3'3
	R (1210) 5'-CCTAGAAGCATTTGCGGTGCACGATG-3'	285	NM_031144	81822
Wnt-4	F (127) 5'-GAAACGTGCGAGAAGCTCAAAG-3'
	R (513) 5'-AAAGGACTGTGAGAAGGCTACG-3'	387	NM_053402	84426
WT-1	F (1059) 5'-TGAGAAACCATACCAGTGTGAC-3'
	R (1458) 5'-GTAGGTGAGAGGGAGGAATTTC-3'	400	NM_031534	24883
Nanog	F (541) 5'-ATCCATTGCAGCTATTCTCAGG-3'
	R (850) 5'-CTTCCAAATTCGCCTCCAAATC-3'	310	XM_575662	414065
AFP	F (1421) 5'-CAGTGAGGAGAAACGGTCCG-3'
	R (1672) 5'-ATGGTCTGTAGGGCTCGGCC-3'	252	NM_012493	24177
Oct-4	F (633) 5'-GGAGATATGCAAATCGGAGACC-3'
	R (984) 5'-CGAGTAGAGTGTGGTGAAATGG-3'	352	NM_001009178	294562
HNF-6	F (1698) 5'-AAGACCAGGACCTCAAGATAGC-3'
	R (2001) 5'-GCAGTGTGGTGGAACAGATAAG-3'	304	NM_022671	25231

¹Wnt-4: Wingless-type mouse mammary tumor virus integration site family, member 4[212627]; WT-1: Wilm’s tumor suppressor gene[1622]; Nanog: One of the gene markers of pluripotency[24]; AFP: α-fetoprotein[1523]; Oct-4: Octomer-4[25]; HNF-6: Hepatic nuclear factor-6[1628];

²Accessed from http://www.ncbi.nlm.nih.gov;

³Numbers in parentheses after forward (F) and reverse primers (R) denote the nucleotide number in the cDNA sequence.