Direct hepatic differentiation of mouse embryonic stem cells induced by valproic acid and cytokines

Table 1 Primers and annealing temperatures used for RT-PCR

Gene	Sequence (5'-3')	Product size (bp)	Annealing temperature (°C)
β-actin-F	TTCCTTCTTGGGTATGGAAT	200	55
β-actin-R	GAGCAATGATCTTGATCTTC
AFP-F	CACTGCTGCAACTCTTCGTA	300	52
AFP-R	CTTTGGACCCTCTTCTGTGA
HNF3β-F	GACCTCTTCCCTTTCTACCG	551	51
HNF3β-R	TTGAAGGCGTAATGGTGC
TTR-F	TGCCTCGCTGGACTGGTAT	334	52
TTR-R	CAGAGTCGTTGGCTGTGAA
DPPIV-F	GATTCCATACCCAAAGGC	587	55
DPPIV-R	GGTCACAACTAAGGCACT
ALB-F	TCTTCGTCTCCGGCTCTG	475	55
ALB-R	CTGGCAACTTCATGCAAA
OCT4-F	GGCGTTCTCTTTGGAAAGGTGTTC	313	57
OCT4-R	CTCGAACCACATCCTTCTCT
AAT-F	AGAACCATTATCAGGCAGAA	675	55
AAT-R	AATAAGGAACGGCTAGTAAGA
DLK-F	GGGGTGACTTCCGTTGC	510	52
DLK-R	GCTCCTCGCCGCTGTTAT
HNF4-F	CTTCCAAGAGCTGCAGATTG	517	55
HNF4-R	CTTGTAGGATTCAGATCCCG
G6P-F	TCAATCTCCTCTGGGTGGC	602	52
G6P-R	GGCAAAGGGTGTAGTGTCAAG
TAT-F	CTTCAGTGCTGGATGTTCGC	619	55
TAT-R	CAGGGATTGGACGGGTTGTT
TDO-F	TAAACAGAGCCAGCAAAG	868	56
TDO-R	ATGAGCGTGTCAATGTCC
BG-F	CGTGAAGGATACGGGAGT	581	55
BG-R	CAGAGTTATTGACGAGGC
GGT-F	TGTCCCTGGTGAAATCCG	577	55
GGT-R	GGCATAGGCAAACCGAAA

AFP: α-fetoprotein; HNF: Hepatocyte nuclear factor; TTR: Transthyretin; DPPIV: Cytokeratin 18; ALB: Albumin; OCT4: Octamer-4; AAT: α-1-antitrypsin; Dlk: Δ-like (Pref-1, preadipocyte factor-1); G6p: Glucose-6-phosphatase; TAT: Tyrosine aminotransferase; TDO: Tryptophan 2,3-dioxygenase; BG: Biliary glycoprotein; GGT: γ-glutamyl transpeptidase.