Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significanly correlated with cytochrome P450 suppression

Table 1 Sequences of oligonucleotide primers

Primer	Sequence
CYP2E1 F	5’-CACCGTTGCCTTGCTTGTCTG-3’
CYP2E1 R	5’-CTCATGAGCTCCAGACACTTC-3’
CYP1A2 F	5’-AGTGTTCTGGATGGTCAGAGC-3’
CYP1A2 R	5’-GCAAGAGGATGCTGACGTCG-3’
GAPDH F	5’-ATGGTGAAGGTCGGTGTGAAC-3’
GAPGH R	5’-GTCTTCTGGGTGGCAGTGATG-3’

Table 2 Effects of TP on CYP450 and CYPb₅ levels (mean ± SD)

Group (n = 10)	CYP450 (nmol/g)	CYPb5 (nmol/g)
Negative control	0.354 ± 0.024b	0.160 ± 0.011b
Low dose	0.279 ± 0.014ab	0.108 ± 0.004ab
Medium dose	0.204 ± 0.016ab	0.077 ± 0.006a
High dose	0.130 ± 0.016a	0.048 ± 0.006a
Positive control	0.147 ± 0.019a	0.061 ± 0.009a

^aP < 0.01 vs negative control group (NS group);

^bP < 0.01 vs positive control group (chloramphenicol group).

Table 3 Effect of TP on mortality induced by paracetamol in mice

Group (n = 10)	Dose (mg/kg)	Death (n)	Death rate (%)	Protection rate (%)
Negative control		9	90	10
Positive control		2	20	80
TP
Low	100	7	70	30
Medium	200	4	40	60
High	400	2	20	80