Copyright
©2008 The WJG Press and Baishideng.
World J Gastroenterol. Dec 14, 2008; 14(46): 7075-7085
Published online Dec 14, 2008. doi: 10.3748/wjg.14.7075
Published online Dec 14, 2008. doi: 10.3748/wjg.14.7075
Table 1 Specifications of the primer sets used for gene expression analysis by RT-PCR
| Gene | Forward primer | Reverse primer |
| IL-1β | CAACAAAAATGCCTCGTGC | TGCTGATGTACCAGTTGGG |
| TNF-α | CATCTTCTCAAAATTCGAGTGACAA | TGGGAGTAGACAAGGTACAACCC |
| MCP-1 | CAGCCAGATGCAGTTAATGCC | AGCCGACTCATTGGGATCAT |
| INOS | GATTTTTCACGACACCCT | GGTCCTCTGGTCAAACTC |
| IL-10 | GTTGCCAAGCCT-TGTCAGAAA | TTTCTGGGCCATGGTTCTCT |
| IFN-γ | CACGCCGCGTCTTGGT | TCTAGGCTTTCAATGAGTGTGCC |
| Suppressors of cytokine signalling proteins | ||
| SOCS3 | CCTCCAGCATCTTTGTC-GGAAGAC | TACTGGTCCAGGAACTCCCGAATG |
| Transcription factors | ||
| T-Bet | TCCTGTCTCCAGCCGTTTCT | CGCTCACTGCTCGGAACTCT |
| GATA-3 | GGCGGCGAGATGGTACTG | TCTGCCCATTCATTTTATGGTAGA |
| Chemokine receptors | ||
| CXCR3 | AGGTCAGTGAACGTCAAGTGCTAG | CAAAAAGAGGAGGCTGTAGAGGA |
| CCR4 | GCCTCCAAGACAGACTTCCTTG | AGCGTTCGGTTCTAGTTTCCAC |
| Housekeeping | ||
| HPRT | GCTCGAGATGTCATGAAGGAGA | TCAGCGCTTTAATGTAATCCAGC |
- Citation: Grémy O, Benderitter M, Linard C. Acute and persisting Th2-like immune response after fractionated colorectal γ-irradiation. World J Gastroenterol 2008; 14(46): 7075-7085
- URL: https://www.wjgnet.com/1007-9327/full/v14/i46/7075.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.7075