First attempt to produce experimental Campylobacter concisus infection in mice

Table 1 C. concisus strains and patient data

Strain	Origin	Diagnosis	No. of experiment	Protein group	AFLP group
ATCC 33237	Oral	Periodontitis	1 and 3	1	1
RH10776.98	Intestinal	Healthy traveller	1 and 3	2	2
RH4204.98	Intestinal	IBD	2 and 3	2	2
RH4482.98	Intestinal	NHL and IBD	2	2	1
RH5097.98	Intestinal	ALL (IR)	2	2	2
RH15690.98	Intestinal	ALL (IR)	2	2	-

NHL: Non Hogdkin Lymphoma; IBD: Inflammatory Bowel Disease; ALL: Acute Lymphoid Leukaemia; IR: In Remission; AFLP: Amplified Fragment Length Polymorphisms.

Table 2 P values based in nominal weight changes from each C. concisus inoculated group compared to controls (n = 12), Weight (g) (P-value)

Day	Control group	Clinical strains		Type strain
		RH10776.981	RH4204.98	ATCC 33237
0	16.0	17.2 (0.60)	16.9 (0.54)	16.6 (0.50)
1	16.4	17.5 (0.57)	16.9 (0.30)	16.6 (0.42)
2	16.7	17.2 (0.20)	16.4 (0.03)	16.6 (0.27)
3	16.8	17.0 (0.13)	16.6 (0.06)	15.4 (0.08)
4	17.3	17.5 (0.17)	16.7 (0.09)	16.5 (0.20)
5	17.5	17.3 (0.04)	16.8 (0.11)	16.6 (0.15)
6	18.1	18.1 (0.12)	17.6 (0.20)	17.4 (0.21)

¹n = 12 from day 5. Weights are expressed as group mean. P-values are calculated using the Mann Whitney U test.

Table 3 Anatomical localization of C. concisus isolation (Experiment 1, day 7, n = 3)

	Stomach	Liver	Ileum	Jejunum	Colon	Stools
Culture	0	3	3	1	0	0
PCR	0	1	1	1	0	0

All isolations were from mice inoculated with the clinical strain RH10776.98.