Identification and characterization of genotype A and D recombinant hepatitis B virus from Indian chronic HBV isolates

Table 1 Primers used for sequencing

Name	Sequence	(nt.)
P1_F	5-TTTTTCACCTCTGCCTAATCA-3	(1821-1841)
SEQ_F1	5-AGGCAACTATTGTGGTTTCA-3	(2194-2212)
SEQ_F2	5-TCTTTAACCCTCATTGGAAA-3	(2516-2535)
SEQ_F3	5-TCACCATATTCTTGGGAACAAGA-3	(2823-2845)
SEQ_F4	5-CTTCCTGCTGGTGGCTCCAGTTC-3	(53-75)
SEQ_F5	5-CTCGTGGTGGACTTCTCTC-3	(253-272)
SEQ_F6	5-ATCCTCAACCACCAGCACG-3	(492-510)
SEQ_F7	5-TATTGGGGGCCAAGTCTGTA-3	(749-768)
SEQ_F8	5-TTTACCCCGTTGCYAGGCA-3	(1144-1162)
SEQ_F9	5-CTCATCTGCCGGACCGTG-3	(1562-1581)
P2_R0	5-AAAAAGTTGCATGGTGCTGG-3	(1825-1841)

Table 2 Baseline characteristics of patients

Patient No.	1	2	3	4	5	6	7	8	9	10	11	12
Age (yr)	8	24	20	20	30	29	88	38	45	40	62	50
Gender	M	F	M	M	M	M	M	F	M	M	M	M
Diagnosis	CHB	CHB	CHB	CHB	CHB	Cirr.	Cirr.	Cirr.	Cirr.	Cirr.	HCC	HCC
Bilirubin (mg/dL)	1.1	0.68	1.1	0.6	1.1	1	0.8	0.4	2.1	1.4	0.9	1.2
ALT (IU/L)	46	54	42	59	52	107	78	29	101	98	31	43
Albumin (g/dL)	4	4.1	4.3	4.8	4.3	4	2.2	3.9	3.0	3.7	4.1	4
PT prolongation (s)	2	2	1	1	2	4	4	18	8	5	2	2
Ascites	No	No	No	No	No	No	No	No	Yes	No	No	No
Encephalopathy	No	No	No	No	No	No	No	No	No	No	No	No
CTPscore	5	5	5	5	5	6	8	7	9	6	5	5
HBeAg	Pos	Pos	Pos	Pos	Pos	Pos	Pos	Neg	Neg	Pos	Pos	Neg
HBV DNA (log copies/mL)	5.4	5.6	6.4	7.2	5.6	6.4	4.9	5.1	5.7	6.1	5.3	6.5
HAI	2	5	6	6	5	-	-	-	-	-	-	-
F Score	1	1	1	3	2	-	-	-	-	-	-	-

Cirr: Cirrhosis; ALT: Alanine aminotransferase; PT: Prothrombin time; CTP: Child-Turcotte-Pugh; HAI; Histological activity index; F: Fibrosis.