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©2008 The WJG Press and Baishideng.
World J Gastroenterol. May 21, 2008; 14(19): 3006-3014
Published online May 21, 2008. doi: 10.3748/wjg.14.3006
Published online May 21, 2008. doi: 10.3748/wjg.14.3006
Table 1 Sequence of primers and amplified length of genes
| Gene | Sequence | Amplified length (bp) |
| RUNX3 | 5’-GAGTTTCACCCTGACCA TCACTGTG-3’ | 870 |
| 5’-GCCCATCACTGGTCTTGAAGGTTGT-3’ | ||
| β-actin | 5’-CTACAATGAGCTGCGTGTGC-3’ | 500 |
| 5’-AGGAGGACTTCTTCTCGAT-3’ |
Table 2 Cell cycle distribution of three groups by FCM (n = 3, mean ± SD, %)
| Groups | G0/G1 | S | G2/M |
| SGC7901 | 43.2 ± 1.2 | 47.7 ± 1.1 | 9.0 ± 1.5 |
| pSilencer3.1-H1-shRNA/SGC7901 | 40.3 ± 2.0 | 49.3 ± 0.9 | 8.1 ± 0.3 |
| pSilencer3.1-H1-shRNA/RUNX3/SGC7901 | 37.2 ± 1.9 | 60.5 ± 0.8 | 7.3 ± 0.9 |
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Citation: Feng XZ, He XS, Zhuang YZ, Luo Q, Jiang JH, Yang S, Tang XF, Liu JL, Chen T. Investigation of transcriptional gene silencing and mechanism induced by shRNAs targeted to
RUNX3 in vitro . World J Gastroenterol 2008; 14(19): 3006-3014 - URL: https://www.wjgnet.com/1007-9327/full/v14/i19/3006.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.3006