Enhanced expressions and activations of leukotriene C4 synthesis enzymes in D-galactosamine/lipopolysaccharide-induced rat fulminant hepatic failure model

Table 1 Primers and application parameters for PCR analysis of the respective genes

Gene	Primer	Designed size (bp)	Annealing temperature (°C)	Mg2+ (mmol/L)	Cycles
LTC4S	Sense 5’-GAAGAACTTTCCACGTGTCG-3’	282	55	2.0	35
	Antisense 5’-GTGCAGCCATTGCCACTAGC-3’
mGST2	Sense 5’-TTCAATCAAGTTTTTGCAACC-3’	204	57	1.5	35
	Antisense 5’-TCTTGGCAACATGAAAGTCC-3’
β-actin	Sense 5’-TGACGGGGTCACCCACACTGTGCCCATCTA-3’	660	58	1.5	28
	Antisense 5’-CTAGAAGCATTTGCGGTGGACGATGGAGGG-3’