Effect of biliary obstruction and internal biliary drainage on hepatic cytochrome P450 isozymes in rats

Table 1 Serum total bilirubin concentration, serum ALT concentration, the total cytochrome P450 content in hepatic microsomes and enzyme activities in hepatic microsomes from sham, bile duct obstruction alone and bile duct obstruction plus drainage groups (mean ± SD, n = 5)

	d 4-sham	d 4-BO	d 10-sham	BOD	d 10-BO
Serum total bilirubin (mg/dL)	0.12 ± 0.04	10.54 ± 3.54a	0.12 ± 0.04	0.16 ± 0.05e	11.18 ± 1.03
Serum ALT (U/L)	36.6 ± 5.7	119.6 ± 50.1a	37.4 ± 3.9	2.5 ± 7.3e	88.8 ± 11.78
Total CYP content (nmol/mg MS protein)	0.577 ± 0.029	0.378 ± 0.012a	0.668 ± 0.080	0.603 ± 0.080e	0.329 ± 0.05
Aniline 4-hydroxylation (nmol/mg MS protein/min)	0.527 ± 0.054	0.261 ± 0.026a	0.568 ± 0.068	0.570 ± 0.091e	0.252 ± 0.03
Aminophylline N-demethylation (nmol/mg MS protein/min)	7.13 ± 0.61	2.83 ± 0.50a	7.88 ± 1.81	5.82 ± 1.00c	2.510 ± 0.23
7-ethoxycoumarin O- demethylation (pmol/mg MS protein/min)	104.4 ± 14.0	68.9 ± 20.5a	123.0 ± 23.8	113.7 ± 22.9e	64.1 ± 4.23

^aP < 0.05 vs d 4-sham;

^cP < 0.05 vs d 4-BO;

^eP < 0.05 vs d 4-BO and d 10-BO; d 10-BO and d 10-sham; BO: Bile duct obstruction alone; BOD: Bile duct obstruction plus drainage; CYP: Cytochrome P450.