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©2008 The WJG Press and Baishideng.
World J Gastroenterol. Mar 14, 2008; 14(10): 1582-1587
Published online Mar 14, 2008. doi: 10.3748/wjg.14.1582
Published online Mar 14, 2008. doi: 10.3748/wjg.14.1582
Table 1 The primer sequences of seven microsatellite markers
| Primer | 3’-5’ | 5’-3’ |
| D1S2877 | AGACATTNCATTGAAGTCTATTTAT | CAAGCCACTAGCGTAAGAGC |
| D1S412 | TAGGACTTTTCAACTTCCACAG | ATAGGCACAGAATCAATGAATG |
| D1S2757 | TTTTTTAATGACTGACCAGTG | TGCCTTCTGCTATGTTTG |
| D1S413 | GCCAAGCCTGAGATCAAAAT | ACTTGAACAGATTGGGATTG |
| D1S2622 | CTGCAACATAAGAACCTAGTGTAAC | AAACTGGTAGGCCATTGATAGA |
| D1S2683 | TGCCTTGTCTTCAAGAGC | GCAGTGACAGGAATCTGG |
| D1S2668 | AATCACTTGAACCTGGGAG | ACTGACTGGCTGTTTCTGAG |
Table 2 LOH frequencies of microsatellite loci on 1q31.1-32.1 in colorectal cancer
| Locus | Location | LOH case | Normal case | LOH rate (%) | Informative rate (%) |
| D1S2877 | 1q31.1 | 7 | 29 | 19.44 | 43.37 |
| D1S412 | 1q31.3 | 11 | 56 | 16.42 | 80.72 |
| D1S2757 | 1q31.3 | 18 | 43 | 29.51 | 73.49 |
| D1S413 | 1q31.3 | 13 | 27 | 32.50 | 48.19 |
| D1S2622 | 1q32.1 | 18 | 31 | 36.73 | 59.04 |
| D1S2683 | 1q32.1 | 10 | 41 | 19.61 | 61.45 |
| D1S2668 | 1q32.1 | 4 | 20 | 16.67 | 28.92 |
Table 3 The relationship between clinicopathological features and LOH of the loci on 1q31.1-32.1
| D1S2877 | D1S412 | D1S2757 | D1S413 | D1S2622 | D1S2683 | D1S2668 | |||||||||
| T1 | N2 | T | N | T | N | T | N | T | N | T | N | T | N | ||
| Gender | Male | 4 | 13 | 3 | 27 | 10 | 18 | 7 | 14 | 11 | 15 | 5 | 20 | 2 | 5 |
| Female | 3 | 16 | 8 | 29 | 8 | 25 | 6 | 13 | 7 | 16 | 5 | 21 | 2 | 15 | |
| Age(yr) | > 60 | 5 | 24 | 9 | 39 | 13 | 32 | 10 | 18 | 16 | 22 | 10 | 29 | 2 | 16 |
| ≤ 60 | 2 | 5 | 2 | 17 | 5 | 11 | 3 | 9 | 2 | 9 | 0 | 12 | 2 | 4 | |
| Location | Proximal colon | 2 | 10 | 6 | 22 | 5 | 18 | 6 | 10 | 9 | 13 | 6 | 13 | 0 | 9 |
| Distal colon | 2 | 8 | 4 | 15 | 5 | 11 | 3 | 7 | 5 | 8 | 0 | 14 | 1 | 7 | |
| Rectum | 3 | 11 | 1 | 19 | 8 | 14 | 4 | 10 | 4 | 10 | 4 | 14 | 3 | 4 | |
| Gross pattern | Massive | 2 | 15 | 5 | 25 | 7 | 22 | 5 | 14 | 6 | 16 | 3 | 22 | 1 | 9 |
| Ulcerative | 4 | 13 | 5 | 23 | 9 | 18 | 5 | 9 | 9 | 14 | 6 | 17 | 1 | 7 | |
| Encroaching | 1 | 1 | 1 | 8 | 2 | 3 | 3 | 4 | 3 | 1 | 1 | 2 | 2 | 4 | |
| Size (cm) | ≥ 5 | 4 | 12 | 7 | 22 | 6 | 18 | 6 | 11 | 8 | 15 | 5 | 17 | 2 | 9 |
| < 5 | 3 | 17 | 4 | 34 | 12 | 25 | 7 | 16 | 10 | 16 | 5 | 24 | 2 | 11 | |
| LN metastasis | (+) | 4 | 20 | 6 | 39 | 12 | 29 | 6 | 16 | 15 | 20 | 5 | 26 | 1 | 16 |
| (-) | 3 | 9 | 5 | 17 | 6 | 14 | 7 | 11 | 3 | 11 | 5 | 15 | 3 | 4 | |
| Differentiation | Well | 3 | 4 | 1 | 14 | 5 | 7 | 3 | 7 | 4 | 5 | 1 | 10 | 3 | 5 |
| Moderately | 2 | 21 | 8 | 26 | 8 | 27 | 6 | 12 | 10 | 18 | 5 | 25 | 0 | 12 | |
| Poorly | 1 | 1 | 2 | 2 | 2 | 2 | 1 | 2 | 1 | 2 | 2 | 1 | 0 | 1 | |
| Mucinous | 1 | 3 | 0 | 14 | 3 | 7 | 3 | 6 | 3 | 6 | 2 | 5 | 1 | 2 | |
| Dukes stage | A | 2 | 5 | 2 | 3 | 3 | 3 | 2 | 3 | 1 | 3 | 2 | 5 | 0 | 1 |
| B | 1 | 4 | 3 | 14 | 3 | 11 | 5 | 8 | 2 | 8 | 3 | 10 | 3 | 3 | |
| C | 3 | 16 | 4 | 29 | 11 | 19 | 4 | 12 | 10 | 17 | 3 | 19 | 0 | 13 | |
| D | 1 | 4 | 2 | 10 | 1 | 10 | 2 | 4 | 5 | 3 | 2 | 7 | 1 | 3 | |
- Citation: Zhou CZ, Qiu GQ, Fan JW, Wang XL, Tang HM, Huang L, Sun YH, Peng ZH. Refined mapping of loss of heterozygosity on 1q31.1-32.1 in sporadic colorectal carcinoma. World J Gastroenterol 2008; 14(10): 1582-1587
- URL: https://www.wjgnet.com/1007-9327/full/v14/i10/1582.htm
- DOI: https://dx.doi.org/10.3748/wjg.14.1582