Uric acid enhances T cell immune responses to hepatitis B surface antigen-pulsed-dendritic cells in mice

Table 1 Surface molecular expression of DCs (%, mean  ±  SD)

Group	n	CD11C	CD83	CD86	MHC
RMPI-1640	5	72.85 ± 1.64	21.66 ± 5.34f	37.77 ± 1.62f	27.34 ± 1.81f
UA100	5	73.16 ± 1.05	47.71 ± 4.75bd	78.48 ± 2.98bf	75.83 ± 2.49bf
UA200	5	73.18 ± 0.95	52.23 ± 0.83b	80.14 ± 1.01b	79.47 ± 0.92b
UA400	5	73.36 ± 1.46	52.33 ± 0.94b	81.08 ± 1.25b	80.36 ± 1.22b
LPS	5	73.44 ± 1.33	53.28 ± 1.12b	82.50 ± 2.29bb	81.42 ± 2.21b

^bP < 0.001 vs RMPI-1640;

^dP < 0.01 vs LPS;

^fP < 0.001 vs LPS.

Table 2 IL-12 p70 production in DC supernatants (mean ± SD, n = 5, ng/mL)

Group	imDC	UA100	UA200	UA400	LPS
IL-12P70	2.53 ± 0.27	3.52 ± 0.22b	3.99 ± 0.28b	4.37 ± 0.19b	4.38 ± 0.17b

^bP < 0.001 vs imDC.

Table 3 IFN-γ production of spleen cells (mean ± SD, n = 10,pg/mL)

Group	IL-4	γ-IFN
PBS	40.598 ± 4.218	135.223 ± 32.563
UA	39.387 ± 3.657	141.500 ± 32.654
Unpulsed-DC	37.352 ± 3.238	149.32 ± 37.354
HBsAg-pulsed-DC	22.385 ± 2.252b	266.575 ± 51.323b
UA-DC	15.123 ± 1.353d	429.216 ± 59.232d

^bP < 0.01,

^dP < 0.001 vs PBS group.

Table 4 T cell proliferative response

Group	n	SI (HBsAg)	SI (PBS)
PBS	10	1.09 ± 0.028	1.062 ± 0.027
UA	10	1.17 ± 0.035	1.067 ± 0.031
Unpulsed-DC	10	1.19 ± 0.055	1.076 ± 0.035
HBsAg-pulsed-DC	10	1.34 ± 0.093b	1.081 ± 0.028
UA-DC	10	1.59 ± 0.156d	1.081 ± 0.029
ConA	10	2.53 ± 0.179d	2.487 ± 0.146

^bP≤ 0.01,

^dP≤ 0.001, vs PBS group.

Table 5 HBsAg-specific CTL cytotoxicity (%, mean ± SD, n = 10)

Group	PBS	UA	Unpulsed-DC	HBsAg-pulsed-DC	UA-DC
Cytotoxicity	3.51 ± 1.14	3.52 ± 1.15	6.32 ± 2.18	27.32 ± 7.32b	68.63 ± 11.32d

^bP≤ 0.01,

^dP≤ 0.001, vs PBS group.