Basic Research
Copyright ©2007 Baishideng Publishing Group Co.
World J Gastroenterol. Feb 14, 2007; 13(6): 882-888
Published online Feb 14, 2007. doi: 10.3748/wjg.v13.i6.882
Table 1 Scoring standard of pathological change for pancreatic tissue of rats with SAP
Pathological gradingPathological changeScores
EdemaInter-lobule local edema, widened pleura1
Inter-lobule diffuse edema, widened intra-lobule clearance2
Increased intra-lobule clearance, alveolus swollen, and separated3
InflammatoryWhite cells < 20/visual field under high-power microscope1
Cell infiltrationWhite cells 20-50/visual field under high-power microscope2
White cells > 30/visual field under high-power microscope, or micro-abscess occurs3
HemorrhageParenchymal hemorrhage < 20%1
Parenchymal hemorrhage 20%-50%2
Parenchymal hemorrhage > 50%3
NecrosisNecrosis area < 20%1
Necrosis area 20%-50%2
Necrosis area > 50%3
Table 2 PCR primer series, position and segment length of NF-κBp65 and β-actin.
GenePrimer series (5'-3')Gene No.PositionLength
NF-κBp65GAAGAAGCGAGACCTGGAGNM_199267424-442398 bp
TCCGGAACACAATGGCCAC821-803
β-actinTCCTAGCACCATGAA GATCNM_0311441044-1062190 bp
AAACGCAGCTCAGTAACAG1233-1215
Table 3 Level of serum amylase at each time point in each group after operation (U/L) n = 10, mean ± SD
GroupsTime points
1 h2 h3 h6 h12 h24 h
NC1835.61491.51530.41400.22153.2337
± 613.2± 507.0± 247.0± 447.5± 236.3± 43.7
SAP2560.52810.54799.34919.73486.32283
± 121.5a± 147.2a± 107.0a± 139.6a± 181.8a± 127.0a
BN2214.53331.741853784.734541360.4
± 109.1b± 196.4b± 147.8bc± 124.1bc± 264.1b± 161.4bc
Table 4 Scores of pancreatic tissues at each time point in each group after operation n = 10, mean ± SD
Groups Time points
1 h2 h3 h6 h12 h24 h
NC0.12 ± 0.050.11 ± 0.060.12 ± 0.050.13 ± 0.040.12 ± 0.060.13 ± 0.05
SAP4.82 ± 0.35a7.65 ± 0.40a8.85 ± 0.39a9.15 ± 0.55a10.10 ± 0.65a11.75 ± 0.25a
BN5.55 ± 0.30b6.65 ± 0.42b5.95 ± 0.19bc5.55 ± 0.36bc6.72 ± 0.30bc9.95 ± 0.58b
Table 5 Absorbance values of NF-κBp65 mRNA in pancreatic tissue at each time in each group after operation n =10, mean ± SD
Groups Time points
1 h2 h3 h6 h12 h24 h
NC220.01 ± 94.51186.30 ± 51.52167.19 ± 51.55114.91 ± 55.96123.91 ± 54.78131.08 ± 66.84
SAP340.47 ± 119.14269.03 ± 66.03a229.45 ± 50.34a197.07 ± 96.02307.42 ± 100.62a376.97 ± 0.22a
BN470.80 ± 122.78bc268.70 ± 87.64b267.95 ± 49.66b277.64 ± 83.37b337.21 ± 103.02b340.24 ± 0.44b
Table 6 Absorbance values of NF-κBp65 protein in pancreatic tissue at each time point in each group after operation. n = 10, mean ± SD
Groups Time points
1 h2 h3 h6 h12 h24 h
NC0.05 ± 0.000.09 ± 0.010.07 ± 0.010.05 ± 0.010.06 ± 0.010.10 ± 0.02
SAP1.43 ± 0.06a0.80 ± 0.05d1.58 ± 0.02a1.38 ± 0.03a0.87 ± 0.09d0.90 ± 0.05d
BN1.04 ± 0.02bc0.72 ± 0.02b1.01 ± 0.04bc0.95 ± 0.03bc0.75 ± 0.02b0.76 ± 0.01b