Rapid Communication
Copyright ©2007 Baishideng Publishing Group Inc.
World J Gastroenterol. Dec 14, 2007; 13(46): 6269-6273
Published online Dec 14, 2007. doi: 10.3748/wjg.v13.i46.6269
Table 1 Oligonucleotide primers
cDNAPrimerSequenceProduct length (bp)
CK20Outer sense5'-GAGGTTCAAC TAACGGAGCT-3'253
Outer antisense5'-TCTCTCTTCCA GGGTGCTTA-3'
Inner sense5'-GCCTTGAGATA GAACTCCAG-3'
Inner antisense5'-ACGTCTTCTCC TTCCAGAAG-3'
GAPDHSense5'-CAGGGCTGCTT TTAACTCTG-3'385
Antisense5'-CTGTTGTCGGAG TTCTAGTAG-3'
Table 2 Comparison between HE and immunohistochemical staining
Vascular invasion
χ2P
(+)(-)
HE staining93719.087< 0.05
Immunohistochemical staining1729
Table 3 Comparative data on vascular invasion
nVI(+)VI(-)χ2P
CK20 mRNA
Positive211296.758< 0.05
Negative25520
Age (yr)
< 50144100.607> 0.05
≥ 50321319
Size (cm)
< 53112190.125> 0.05
≥ 515510
Differentiated
Well11292.351> 0.05
Moderately20812
Poorly1578
Serosa invasion
Negative142124.440< 0.05
Positive321517
Lymph node metastasis
Negative183155.225< 0.05
Positive281414
Distant metastasis
Negative3892916.520< 0.05
Positive88
Table 4 Average number of microvessels of tissue in VI positive and negative patients
nMVDtP
VI
Positive1729.2 ± 3.32.987< 0.05
Negative2925.4 ± 4.7