Analysis of immune responses against H pylori in rabbits

Table 1 Serum antibody response in rabbit to H pylori whole cell antigen detected by in-house ELISA

		Dilution of sera
Serum collection	Neat	1:50	1:100	1:200	1:400	1:800
d 0	0.998	0.847	0.620	0.063	0.024	Not done
d 7	2.777	2.562	2.029	1.835	1.245	1.028
d 14	2.003	3.136	2.792	2.241	0.408	1.404
d 21	3.004	3.267	2.857	3.303	2.973	1.974
d 27	2.805	2.894	2.021	2.074	2.323	1.004
d 35	2.62	2.958	3.128	2.947	3.112	2.857
d 42	3.002	2.945	2.763	2.719	2.999	3.303

Table 2 Serum antibody responses of subjects to H pylori antigens detected by immunoblotting and in-house ELISA

Collection of serumsamples	Sera with antibodiesby immunoblotting1	Sera with antibodiesby ELISA2
d 0	-	-
d 7	+	+
d 14	+	+
d 21	+	+
d 27	+	+
d 35	+	+
d 42	+	+

¹In immunoblotting more than 4 protein bands indicated positive results;

²When ELISA values were ≥ 1.0, OD₄₀₅ was considered positive results.