Rationales for expression and altered expression of apoptotic protease activating factor-1 gene in gastric cancer

Table 1 Polymorphic sites, primers and amplification conditions (12q22-23)

Site	Repeat	Heterozygosity	Amplification length (kb)	Primer sequence	Reaction conditions
D12S327	Dinucleotide	0.8501	0.1820-0.2010	AAA GTT TCT GGA TGG TAA TAT CG	54°C 40 s
				AGA GCA AGA CCT TGT CTC AA	72°C 45 s
D12S1657	Dinucleotide	0.6439	0.1500-0.1600	TCC TAA AGA TGG TGT GCA T	58°C 40 s
				AAG TTC CAA TGT TAG TGA ACC	72°C 45 s
D12S393	Tetranucleotide	0.6410	0.2490	ATT ATT GCC AGG ACA TTA AAC G	58°C 30 s
				CCT CAC ACA ATG TTG TAA GGG	72°C 40 s
D12S1706	Dinucleotide	0.8916	0.1190-0.1390	CCT ATG ATT TCC CAT CAA GTT T	57°C 40 s
				ATT ATT AGG AGA GCC CTG GG	72°C 40 s
D12S346	Dinucleotide	0.8400	0.1660	TGC CC ACCT GCC TGT AAC	58°C 30 s
				AAT GGA GGG TAA ATG CCC G	72°C 40 s

Table 2 Correlation of Apaf-1, LOH and Apaf-1 mRNA expression in gastric cancer tissue samples

		mRNA expression of Apaf-1 gene (n)		rS	P
		Down-regulation	Up-regulation
More than 2 sites (n)	+	13	5	0.487	0.003
	-	4	13

Table 3 Correlation of Apaf-1 methylation and Apaf-1 mRNA expression in gastric cancer tissue samples

		mRNA expression of Apaf-1 (n)		rS	P
		Down-regulation	Up-regulation
Apaf-1 gene	+	16	1	0.886	10-6
methylation (n)	-	1	17